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Bilirubin (liquid)
| Catalog # | Name | Size | Price | Qty | |
|---|---|---|---|---|---|
| BR411 |
Bilirubin (liquid)
Method: Jendrassik |
1 x 225 or 2 x 625ml | £25.73 |
Specification
| Format: | Liquid ready to use |
| Assay Range : | Total Bilirubin 1.83 - 560µmol/l, Direct Bilirubin 0.872 - 608µmol/l |
| Working Stability 15-25 °C: | Stable to expiry |
| Working Stability 2-8 °C: | _ |
| Request Kit Insert |
Enquiry / Requests
Payment Options
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Offline: Please call us on +44 (0) 28 9442 2413 or email us at marketing@randox.com
Intended Use
For the quantitative in vitro determination of total and direct bilirubin in serum or plasma. This product is suitable for both automated and manual use.
Clinical Significance
Bilirubin is formed by the breakdown of haemoglobin in the spleen, liver and bone marrow. In the liver, bilirubin is conjugated with glucuronic acid to form a soluble compound. This conjugated bilirubin passes down the bile duct and is excreted into the gastrointestinal tract. An unconjugated, albumin bound form is also present in the circulation. It is insoluble and does not normally pass through the kidneys into the urine.
An increase in bilirubin concentration in the serum or tissues can lead to a condition called jaundice. Jaundice occurs in toxic/infectious diseases of the liver e.g. hepatitis B, obstruction of the bile duct and in rhesus incompatible babies.
Too much bilirubin in the blood can indicate that the red blood cells are being destroyed too quickly or that the liver can not remove all of the bilirubin from the blood. In newborn babies excess bilirubin can lead to retardation or physical abnormalities, early detection is therefore extremely important.
Useful information may be obtained by determining which form of bilirubin is elevated. High levels of conjugated or direct bilirubin indicate that bile is not being properly excreted; therefore an obstruction may be present in the bile duct or gall bladder. Unconjugated or indirect bilirubin can also be determined by subtracting the direct bilirubin level from the total bilirubin result. High levels of unconjugated bilirubin indicate that too much haemoglobin is being destroyed or that the liver is not actively treating the haemoglobin it is receiving.
Principle
Colorimetric method based on that described by Jendrassik and Grof (1938). Direct (conjugated) bilirubin reacts with diazotised sulphanilic acid in alkaline medium to form a blue coloured complex. Total bilirubin is determined in the presence of caffeine, which releases albumin bound bilirubin, by the reaction with diazotised sulphanilic acid.