Bilirubin assay
| Product | Method | Size | Catalog | Price | Quantity |
| Bilirubin assay | DPD | R1 6x50ml, R2 3x20ml | BR7939 | £228.75 | |
| Shipping costs will be added at the checkout stage, click here for charges. | |||||
- Format
Lyophilised - Assay Range
5.2 - 606µmol/l - Working Stability 15-25 °C
5 days - Working Stability 2-8 °C
10 days - Material Safety Data Sheets
Intended Use
For the quantitative in vitro determination of Bilirubin in Serum or Plasma. This product is suitable for use on the Hitachi 704, 717, 902 and Hitachi 911/912.
Clinical Significance
Bilirubin is formed by the breakdown of haemoglobin in the spleen, liver and bone marrow. In the liver, bilirubin is conjugated with glucuronic acid to form a soluble compound. This conjugated bilirubin passes down the bile duct and is excreted into the gastrointestinal tract. An unconjugated, albumin bound form is also present in the circulation. It is insoluble and does not normally pass through the kidneys into the urine.
An increase in bilirubin concentration in the serum or tissues can lead to a condition called jaundice. Jaundice occurs in toxic/infectious diseases of the liver e.g. hepatitis B, obstruction of the bile duct and in rhesus incompatible babies.
Too much bilirubin in the blood can indicate that the red blood cells are being destroyed too quickly or that the liver can not remove all of the bilirubin from the blood. In newborn babies excess bilirubin can lead to retardation or physical abnormalities, early detection is therefore extremely important.
Useful information may be obtained by determining which form of bilirubin is elevated. High levels of conjugated or direct bilirubin indicate that bile is not being properly excreted; therefore an obstruction may be present in the bile duct or gall bladder. Unconjugated or indirect bilirubin can also be determined by subtracting the direct bilirubin level from the total bilirubin result. High levels of unconjugated bilirubin indicate that too much haemoglobin is being destroyed or that the liver is not actively treating the haemoglobin it is receiving.
Principle
Bilirubin reacts with a dichlorophenyl diazonium compound (DPD) to form a coloured azobilirubin. Indirect bilirubin is liberated by a detergent.
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