Alt (gpt) assay
| Product | Method | Size | Catalog | Price | Quantity |
| Alt (gpt) assay | IFCC with P5P | 240T | AL2808 | £92.50 | |
| Shipping costs will be added at the checkout stage, click here for charges. | |||||
- Format
Mixing and diluting are automatically performed by the DIMENSIONâ instrument. - Assay Range
53.5 - 1118U/L - Working Stability 15-25 °C
_ - Working Stability 2-8 °C
Stable to expiry - Material Safety Data Sheets
Intended Use
For the quantitative in vitro determination of alanine aminotransferase activity in serum or plasma. This product is suitable for use on Siemens Dimension analysers.
Clinical Significance
The aminotransferases are a group of enzymes that catalyse the inter-conversion of amino acids and a oxoacids by transferring amino groups. The enzyme ALT (alanine aminotransferase or glutamate pyruvate transaminase) has been found to be in highest concentrations in the liver, with decreasing concentrations found in the kidneys, heart, skeletal muscle, pancreas, spleen and lung tissue respectively .
ALT measurements are used in the diagnosis and treatment of certain liver diseases (e.g. viral hepatitis and cirrhosis) and heart diseases. Extremely elevated levels of ALT can indicate acute hepatitis with moderately high levels indicating chronic hepatitis.
Elevated levels of the transaminases can also be indicative of myocardial infarction, hepatic disease, muscular dystrophy and organ damage. Elevated levels of ALT in serum is rare except in parenchymal liver disease, since ALT is a more liver specific enzyme.
ALT is often tested in combination with AST as part of a liver panel with ALT levels being higher in most types of liver disease.
Principle
Alanine aminotransferase (ALT) catalyzes the transamination of L-alanine to α-ketoglutarate (α-KG), forming L-glutamate and pyruvate. The pyruvate formed is reduced to lactate by lactate dehydrogenase (LDH) with simultaneous oxidation of reduced nicotinamide-adenine dinucleotide (NADH). The change in absorbance is directly proportional to the ALT activity and is measured using a bichromatic (340, 700 nm) rate technique.
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