Ammonia assay
| Product | Method | Size | Catalog | Price | Quantity |
| Ammonia assay | Enzymatic UV | 380T | AM3979 | £120.73 | |
| Shipping costs will be added at the checkout stage, click here for charges. | |||||
- Format
Lyophilised - Assay Range
23.4 - 1032µmol/l - Working Stability 15-25 °C
5 days - Working Stability 2-8 °C
3 weeks - Material Safety Data Sheets
Intended Use
For the quantitative in vitro determination of Ammonia in plasma (for biotechnology use only). This product is suitable for use on RX Series instruments which includes the Rx Daytona and the Rx Imola.
Clinical Significance
The major source of circulating ammonia is the GI tract. Under normal conditions, ammonia is metabolised to urea by liver enzymes. Several diseases, both inherited and acquired can result in significantly elevated levels of ammonia in the blood (hyperammonemia).
Inherited deficiencies or defects of urea cycle enzymes is the major cause of hyperammonemia in infants however extremely high levels of ammonia may also be associated with haemolytic disease in newborn babies.
Reye’s syndrome an acquired hyperammonemia disease is characterised by high levels of ammonia and decreased levels of glucose as such ammonia is usually tested alongside glucose, electrolytes, kidney and liver function tests in adolescents and children showing symptoms.
High levels of ammonia in the blood are also associated with both liver disease and renal failure. Elevated ammonia is toxic to the central nervous system.
Principle
Ammonia combines with αketoglutarate and NADPH in the presence of glutamate dehydrogenase (GLDH) to yield glutamate and NADP+. The corresponding decrease in absorbance at 340 nm is proportional to the plasma ammonia concentration.
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