Glutamine assay
| Product | Method | Size | Catalog | Price | Quantity |
| Glutamine assay | Colorimetric | R1 4x16, R2 4x6.7ml | GM1373 | £701.50 | |
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- Format
Lyophilised - Assay Range
0.14 - 10mmol/l - Working Stability 15-25 °C
_ - Working Stability 2-8 °C
7 days - Material Safety Data Sheets
Intended Use
For the quantitative determination of L-glutamine for Biotechnology use only. This product is suitable for use the RX Series instruments which includes the Rx Daytona and Rx Imola.
Principle
L-glutamine in cell culture is determined spectrophoto-metrically through a kinetic coupling assay system using glutaminase and L-glutamate oxidase. L-glutamate generated from deamination of L-glutamine is oxidized by L-glutamate oxidase to release H2O2. H2O2 is further reacted with N-Ethyl-N-(2-hydroxy-3-sulfo-propyl)-3-methylaniline (EHSPT) and 4-aminoantipyrine (4-AA) in the presence of peroxidase (POD) to generate quinine dye which is proportional to the amount of L-glutamine in samples.
- Various
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