Theophylline assay
| Product | Method | Size | Catalog | Price | Quantity |
| Theophylline assay | LEI | R1 2 x 17ml, R2 2 x 5ml | TD3412 | £368.30 | |
| Shipping costs will be added at the checkout stage, click here for charges. | |||||
- Format
Liquid ready to use - Assay Range
1.64 - 42.7 - Working Stability 15-25 °C
_ - Working Stability 2-8 °C
Stable to expiry - Material Safety Data Sheets
Intended Use
For the quantitative in vitro determination of Theophylline in human serum. This product is suitable for use on the RX Series instruments which includes the Rx Daytona and Rx Imola.
Clinical Significance
It is essential to maintain a constant concentration of medication in order for treatment to be successful, insufficient levels can mean drugs are not effective and excessive levels in some cases can lead to toxicity.
Often there is little difference between the dosage required for effective treatment and the dosage needed to cause severe side effects and even toxicity. The fact that individuals absorb, metabolise and make use of drugs at different rates means a standard dose cannot be prescribed for everyone. It is therefore extremely important to monitor the use of drugs with a narrow therapeutic range to ensure individuals are receiving the optimum treatment.
Theophylline is a bronchodilator used to treat asthma and other airway diseases. Measurements obtained are used in the diagnosis and treatment of theophylline overdose or in monitoring levels of theophylline to ensure appropriate therapy.
Principle
Randox Theophylline assay is a latex-enhanced immunoturbidimetric assay. It is based on the principle of measuring changes in scattered light. The latex particles are coated with theophylline, which in the presence of theophylline antibody solution, rapidly agglutinate.
When a sample containing theophylline is introduced, the agglutination reaction is partially inhibited, slowing down the agglutination process. The rate of agglutination is inversely dependent upon the concentration of theophylline in the sample. By monitoring the change in scattered light, as a change in absorbance, a concentration curve can be obtained. The actual change in absorbance is inversely proportional to the concentration of theophylline in the sample.
This assay uses an end point method and multipoint calibration.
- Various
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