Ahd ELISA
| Product | Method | Size | Catalog | Price | Quantity |
| Ahd ELISA | ELISA | 96T | NF3463 | £918.75 | |
| Shipping costs will be added at the checkout stage, click here for charges. | |||||
- Material Safety Data Sheets
Intended Use
To be used in combination with the Nitrofurans Accessory kit, for the quantitative in vitro determination of Nitrofurantoin (i.e. AHD) in a test sample i.e. tissue (prawn, beef, pork and poultry).
General Background
Nitrofurans have been widely used in veterinary practice as antibacterial agents in the treatment of pigs, poultry, fish and shrimp. Bans on nitrofurans were introduced within the EU primarily because of concerns about their carcinogenicity and mutagenicity. Several other Non-European countries have also banned all uses of nitrofurans in food producing animals.
Tissue residue of Nitrofurantoin is mainly protein bound, therefore the main metabolite 1-aminohydantoin (AHD) is proposed as the marker residue.
This ELISA provides a fast, reliable, analytical method to detect the presence of Nitrofurans. If performing the test using duplicate wells, this kit has the ability to analyse 40 samples per plate.
Principle
Each microtitre plate is supplied precoated with an AHD antibody. AHD (antigen) in the Nitrofuran standard (multi antigen), and if present in the sample, competes with the horseradish peroxidase labelled AHD in the Nitrofuran multi conjugate for a limited number of antibody sites on the microtitre plate. After incubation at room temperature, to allow a competition reaction to take place, the microtitre plate is washed to remove excess reagents.
The enzyme substrate is added. After an incubation period to allow maximum colour development, the colour reaction is stopped by the addition of acid. This produces a colour change from blue to yellow, and the absorbances are read at 450nm.
A standard curve is then constructed to determine the AHD concentration in the sample.
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