Beta-lactam ELISA
| Product | Method | Size | Catalog | Price | Quantity |
| Beta-lactam ELISA | ELISA | 96T | BL3448 | £1147.00 | |
| Shipping costs will be added at the checkout stage, click here for charges. | |||||
- Material Safety Data Sheets
Intended Use
For the quantitative in-vitro determination of nafcillin, ampicillin, amoxicillin, dicloxacillin, cloxacillin, oxacillin, penicillin G, penicillin V, piperacillin, metampicillin and azlocillin as ampicillin equivalence in milk, urine, tissue (liver, kidney and muscle), serum and plasma samples.
Clinical Significance
Beta-lactam antibiotics are among the most widely used antibiotics for the treatment of bacterial infections in man and animals. Beta-lactams are also administered to animals in feed for growth promotion and for collective prophylactic treatment. The monitoring of beta-lactam residues in edible tissues and milk is important because of the hypersensitivity of some individuals to these antibiotics and also the emergence of antibiotic-resistant strains of bacteria. For this reason, maximum residue limits have been specified for food products and milk to try and control the levels of these antibiotics reaching the consumer.
This ELISA aims to give a fast, reliable, analytical method to detect the presence of beta-lactams. This kit can be used as a screening method for the presence of beta-lactams and will give results in 1½ hours (not including sample and reagent preparation). If performing the test using duplicate wells, this kit has the ability to analyse 40 samples.
Principle
A microtitre plate is supplied pre-coated with a beta-lactam antibody. Beta-lactams (antigen), if present in the standard and sample compete with horseradish peroxidase labelled beta-lactam (enzyme labelled antigen) for a limited number of antibody sites on the microtitre plate. After incubation at room temperature to allow a competition reaction to take place, the microtitre plate is then washed to remove excess reagents.
The enzyme substrate is added. After an incubation period to allow maximum colour development, the colour reaction is stopped by addition of acid. This produces a colour change from blue to yellow, and the absorbances are read at 450 nm.
A standard curve is then constructed to determine the beta-lactam concentration in the standard and sample.
Related Products
We accept all major credit cards
You can also buy via phone or email
+44 (0) 9442 2413
marketing@randox.com
