Corticosteroid ELISA
| Product | Method | Size | Catalog | Price | Quantity |
| Corticosteroid ELISA | ELISA | 96T | DM2156 | £1147.00 | |
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- Material Safety Data Sheets
Intended Use
For the quantitative in vitro determination of dexamethasone, flumethasone and betamethasone in urine and tissue (muscle, liver and kidney) samples.
Clinical Significance
Corticosteroids are a group of natural and synthetic analogues of the hormones secreted by the pituitary gland. These include glucocorticoids, such as dexamethasone, flumethasone and β-methasone.
Synthetic glucocorticoids are commonly used in veterinary medicine as anti-inflammatory agents, and illicit use as growth-promoters is suspected. Dexamethasone is rapidly absorbed and excreted in urine and faeces, primarily as the parent molecule, tracer and inactive 6-hydroxy metabolites.
Corticosteroids are licensed in several countries for a range of veterinary uses. The European Union has set MRLs for dexamethasone and betamethasone of 0.3ppb in bovine milk, 2ppb in bovine and porcine liver and 0.75ppb in bovine and porcine muscle and kidney.
Principle
A microtitre plate is pre-coated with an antibody specific to dexamethasone, flumethasone and betamethasone. Corticosteroids, present in standards or samples, compete for antibody sites with a horseradish peroxidase-labelled corticosteroid conjugate.
The microtitre plate is then washed and the enzyme substrate is added.
Following the recommended incubation period at room temperature, the colour reaction is stopped by the addition of Stop Solution.
The optical density is read at 450 nm with the colour intensity being inversely proportional to the concentration of corticosteroid in the standard or sample.
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