Ketamine ELISA
| Product | Method | Size | Catalog | Price | Quantity |
| Ketamine ELISA | ELISA | 96T | KT3459 | £1043.00 | |
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- Material Safety Data Sheets
Enzyme-Linked Immunosorbent Assay
Intended Use
For the quantitative in vitro determination of Ketamine in urine (equine).
General Background
Ketamine was first synthesized in 1962 and subsequently marketed as an anaesthetic drug for both human and animal use. It is a dissociative medical anaesthetic agent possessing hallucinogenic properties.
Ketamine became a drug of abuse shortly after it was introduced to clinical use. It acts by depressing the central nervous system causing a short lasting loss of body sensation. It is metabolised in the liver by N-demethylation to produce the active metabolite Norketamine.
As well as analgesic properties Ketamine also possesses hallucinogenic properties which have led to its abuse worldwide. Ketamine is a class C drug meaning it is illegal to both possess and supply it as such it has been placed on the screening list for illicit drugs.
Detecting the miss use of Ketamine is important in many fields including forensic toxicology, medico-legal applications, sports doping and pharmaceutical research.
Principle
A micro titre plate is supplied precoated with ketamine antibody. Ketamine (antigen), if present in the sample competes with the horseradish peroxidase labelled Ketamine (enzyme labelled antigen) for a limited number of antibody sites on the microtitre plate. After incubation at room temperature to allow a competition reaction to take place, the microtitre plate is washed to remove excess reagents.
The enzyme substrate is added. After an incubation period to allow maximum colour development, the colour reaction is stopped by the addition of acid. This produces a colour change from blue to yellow, and the absorbance’s are read at 450nm.
A standard curve is then constructed to determine the ketamine concentration in the standard and sample.
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