Leucomalachite green ELISA
| Product | Method | Size | Catalog | Price | Quantity |
| Leucomalachite green ELISA | ELISA | 96T | LMG3466 | £1043.00 | |
| Shipping costs will be added at the checkout stage, click here for charges. | |||||
Intended Use
For the quantitative in vitro determination of triphenylmethane dyes; leucomalachite green, malachite green and leucocrystal violet, in fish tissue.
General Background
Malachite green is a triphenylmethane dye which is used extensively in the aquaculture industry for the treatment of fungal and protozoal infections. (1). The toxicity of the dye increases with exposure time, temperature and concentration and has been reported to cause carcinogenesis, mutagenesis, chromosomal fractures, teratogenicity and respiratory toxicity (2). Malachite green has a high level of abuse due to its efficacy and the lack of equally effective alternatives. (3) It is easily absorbed during waterborne exposure (3) and metabolised to the colourless compound, leucomalachite green, which can persist in fatty fish tissue for long periods of time. (4).
This ELISA aims to give a fast, reliable, analytical method to detect the presence of leucomalachite green, malachite green and leucocrystal violet. This kit can be used as a screening method for the presence of three triphenylmethane dyes and will give results in less than 2½ hours (not including sample and reagent preparation). If performing the test using duplicate wells, this kit has the ability to analyse 40 samples.
Principle
A microtitre plate is supplied precoated with leucomalachite green antibody. Triphenylmethane dyes (antigen), if present in the standard and sample compete with horseradish peroxidase labelled leucomalachite green (enzyme labelled antigen) for a limited number of antibody sites on the microtitre plate. After incubation at room temperature to allow a competition reaction to take place, the microtitre plate is washed to remove excess reagents.
The enzyme substrate is added. After an incubation period to allow maximum colour development, the colour reaction is stopped by addition of acid. This produces a colour change from blue to yellow, and the absorbances are read at 450 nm (ref 630nm).
A standard curve is then constructed to determine the leucomalachite green concentration in the standard and sample.
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