Nitrofurans accessory kit
| Product | Method | Size | Catalog | Price | Quantity |
| Nitrofurans accessory kit | 384T | NF3464 | £190.52 | ||
| Shipping costs will be added at the checkout stage, click here for charges. | |||||
- Material Safety Data Sheets
Intended use
To be used in combination with one of the Nitrofuran ELISA Kits, for the quantitative in vitro determination of Nitrofurans i.e. Nitrofurantoin (AHD), Furazolidone (AOZ), Furaltadone (AMOZ) and Nitrofurazone (SEM) in a test sample i.e. tissue (prawn, beef, pork and poultry).
General Background
Nitrofurans have been widely used in veterinary practice as antibacterial agents in the treatment of pigs, poultry, fish and shrimp. The uses of nitrofurans are prohibited within the EU primarily because of concerns about their carcinogenicity and mutagenicity. Several other non-European countries have also banned the use of nitrofurans in food producing animals.
Nitrofurantoin, Furazolidone, Furaltadone and Nitrofurazone are the four main nitrofuran antibiotics. Studies have shown them to be rapidly metabolised by animals in vivo, however the tissue bound metabolites formed, 1-aminohydantoin (AHD), 3amino-2-oxazlidinone (AOZ), 3amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) and Semicarbazide (SEM) can persist for at least 6 weeks. Therefore these metabolites are proposed as the marker residues.
This ELISA provides a fast, reliable, analytical method to detect the presence of Nitrofurans. If performing the test using duplicate wells, this kit has the ability to analyse 40 samples per plate.
Principle
Each microtitre plate is supplied precoated with a Nitrofuran antibody. Depending on the Nitrofuran being tested, the appropriate plate should be used (refer to flow chart), i.e. if testing for the presence of AHD then the AHD plate should be used. Nitrofuran (antigen) in the standard (multi antigen) and, if present in the sample, competes with the horseradish peroxidase labelled Nitrofuran (multi conjugate) for a limited number of antibody sites on the microtitre plate. After incubation at room temperature, to allow a competition reaction to take place, the microtitre plate is washed to remove excess reagents.
The enzyme substrate is added. After an incubation period to allow maximum colour development, the colour reaction is stopped by the addition of acid. This produces a colour change from blue to yellow, and the absorbances are read at 450nm.
A standard curve is then constructed to determine the Nitrofuran concentration in the sample.
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