Quinolones ELISA
| Product | Method | Size | Catalog | Price | Quantity |
| Quinolones ELISA | ELISA | 96T | QL3454 | £1147.00 | |
| Shipping costs will be added at the checkout stage, click here for charges. | |||||
- Material Safety Data Sheets
Intended Use
For the quantitative in vitro determination of nadifloxacin, rufloxacin, danofloxacin, enrofloxacin, ciprofloxacin, levofloxacin, ofloxacin, pefloxacin, prulifloxacin, norfloxacin, difloxacin, sarafloxacin, enoxacin, marbofloxacin, fleroxacin, clinafloxacin and lomefloxacin in milk and tissue.
General Background
Quinolones are a group of synthetic antimicrobials that inhibit the activity of bacterial DNA gyrase enzymes. The earliest members of this group of compounds are active against gram-negative bacteria. The newer fluoroquinolone antibiotics, a fluorine-containing subclass of the quinolones, have been found to have enhanced antibacterial activity against gram-negative bacteria and they are also active against gram-positive bacteria.
Quinolones are mainly used to treat infections, including, gastrointestinal and respiratory infections in both human and veterinary clinical practices. However, the presence of residues of these compounds in food producing animals has to be controlled as their widespread use can lead to the emergence of antibiotic resistant bacteria. As such maximum residue limits have been set for several quinolones depending on animal species and matrices.
This ELISA aims to give a fast, reliable, analytical method to detect the presence of fluoroquinolones. This kit can be used as a screening method for the presence of seventeen fluoroquinolones and will give results in 1½ hours (not including sample and reagent preparation). If performing the test using duplicate wells, this kit has the ability to analyse 40 samples.
Principle
A micro titre plate is supplied precoated with norfloxacin antibody. Fluoroquinolones (antigen), if present in the standard and sample compete with horseradish peroxidase labelled norfloxacin (enzyme labelled antigen) for a limited number of antibody sites on the microtitre plate. After incubation at room temperature to allow a competition reaction to take place, the microtitre plate is washed to remove excess reagents.
The enzyme substrate is added. After an incubation period to allow maximum colour development, the colour reaction is stopped by addition of acid. This produces a colour change from blue to yellow, and the absorbances are read at 450 nm.
A standard curve is then constructed to determine the fluoroquinolone concentration in the standard and sample.
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