Ractopamine ELISA
| Product | Method | Size | Catalog | Price | Quantity |
| Ractopamine ELISA | ELISA | 96T | RT3451 | £1147.00 | |
| Shipping costs will be added at the checkout stage, click here for charges. | |||||
- Material Safety Data Sheets
Intended Use
This kit quantitatively measures the level of Ractopamine in urine, tissue samples and animal feeds.
General Background
Ractopamine is a phenethanolamine belonging to a class of drugs known as β-Adrenergic agonists (beta agonists). The β-agonists are a group of compounds that have a stimulatory effect on the b-adrenergic receptors and have been used in both human and veterinary medicine as bronchodilatory agents.
Ractopamine as well as other β-Agonists have been found to increase muscle growth and decrease fat deposition and as such has a history of being used illegally, particularly by livestock producers.
Improper use of beta agonists can cause a serious risk to human health due to the residues they leave behind in meat and other foodstuffs of animal origin. It is for this reason that in Europe all beta agonists are banned for use in livestock and for improving athletic performance according to EU council directive 96/22/EC.
This ELISA aims to give a fast reliable analytical method to detect the presence of Ractopamine (racemic). This kit can be used as a screening method for the presence of Ractopamine and will give results in 1½ hours (not including sample preparation). If performing the test using duplicate wells, this kit has the ability to analyse 40 samples.
Principle
A microtitre plate is supplied precoated with a ractopamine antibody. Ractopamine (antigen), if present in the standard and sample competes with horseradish peroxidase labelled ractopamine (enzyme labelled antigen) for a limited number of antibody sites on the microtitre plate. After incubation at room temperature to allow a competition reaction to take place, the microtitre plate is then washed to remove excess reagents.
The enzyme substrate is added. After an incubation period to allow maximum colour development, the colour reaction is stopped by addition of acid. This produces a colour change from blue to yellow, and the absorbances are read at 450 nm. A standard curve is then constructed to determine the ractopamine concentration in the standard and sample.
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