Stilbene ELISA
| Product | Method | Size | Catalog | Price | Quantity |
| Stilbene ELISA | ELISA | 96T | SJ2152 | £1147.00 | |
| Shipping costs will be added at the checkout stage, click here for charges. | |||||
- Material Safety Data Sheets
Intended Use
For the quantitative in vitro determination of hexestrol, diethylstilbestrol and dienestrol in bile, tissue, serum and urine samples. This assay is carried out in a simple 2 step format. Standards used in this kit are an alcohol and water mix, please ensure that the instructions documented in this procedure are followed.
General Background
The stilbene derivatives diethylstilbestrol (DES), dienestrol and hexestrol are synthetic oestrogens that have been demonstrated to be genotoxic and carcinogenic in both animals and humans.
Diethylstilbestrol (DES) has been used commercially as a growth-promoting agent in livestock, as a human medication to help maintain pregnancy and in the treatment of prostate cancer in humans, but was removed from these uses due to human carcinogenicity issues.
DES, dienestrol and hexestrol are excreted in urine primarily as glucuronide tracer.
The European Union has prohibited the use of stilbenes and stilbene derivatives in food producing animals; meaning that any detection of stilbenes would be a violation. The European Union is currently working towards specifying MRPLs for assays for prohibited substances.
Principle
A micro titre plate is supplied precoated with an antibody cross-reactive for hexestrol, diethylstilbestrol and dienestrol. The assay is carried out in a 2 step format. Standards (Hexestrol, alcohol/water mixture) and samples are added to the microtitre plate and incubated for 1 hour at room temperature (+19 to +25°C). After this incubation an enzyme labelled stilbene is added and the plate incubated for a further 30 minutes at room temperature (+19 to +25°C). The micro titre plate is then washed and enzyme substrate and chromogen are added. Following an incubation period to allow maximum colour development the colour reaction is stopped and the absorbances determined. A standard curve is then constructed in order to determine the concentration of hexestrol, diethylstilbestrol or dienestrol in the bile, urine, serum or tissue sample.
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