Sulphadiazine ELISA
| Product | Method | Size | Catalog | Price | Quantity |
| Sulphadiazine ELISA | ELISA | 96T | SZ2147 | £894.25 | |
| Shipping costs will be added at the checkout stage, click here for charges. | |||||
- Material Safety Data Sheets
Intended Use
For the quantitative in vitro determination of Sulphadiazine in tissue, urine, milk, egg and serum samples. This product is suitable for Manual use.
General Background
The sulphonamides (i.e. sulphamethoxazole) are competitive inhibitors of the enzyme dihydropteroate synthetase in the folic acid metabolism cycle, which inhibit bacterial growth by preventing p-aminobenzoic acid incorporation into folic acid. The sulphonamides have a bacteriostatic effect on a broad range of gram-positive and gram-negative organisms.
Sulphonamides are also widely used as growth promoters in calves and pigs, residues may therefore occur in foodstuff of animal origin. Sulphadiazine is carcinogenic and can therefore pose a threat to human health.
Principle
Antibody to Sulphadiazine is bound to a microtitre plate. Sulphadiazine in the sample competes with the horseradish peroxidase labelled Sulphadiazine (enzyme-labelled antigen) for binding to the limited number of antibody sites on the microtitre plate. The enzyme substrate is added. After an appropriate time has elapsed for maximum colour development, the enzyme reaction is stopped by the addition of acid producing a colour change from blue to yellow. The optical density is read at 450 nm with the colour intensity being inversely proportional to the concentration of sulphadiazine in the sample.
We accept all major credit cards
You can also buy via phone or email
+44 (0) 9442 2413
marketing@randox.com
