Sulphamethazine ELISA
| Product | Method | Size | Catalog | Price | Quantity |
| Sulphamethazine ELISA | ELISA | 96T | SM2146 | £894.25 | |
| Shipping costs will be added at the checkout stage, click here for charges. | |||||
- Material Safety Data Sheets
Intended Use
For the quantitative in vitro determination of Sulphamethazine (SMT) in tissue, urine, milk, egg and serum samples. This product is suitable for Manual use.
Background Information
Sulphamethazine (Sulfamethazine) belongs to a group of antibiotics collectively known as the sulphonamides. Sulphamethazine is used in veterinary medicine to treat a variety of bacterial diseases including respiratory disease. Sulphamethazine has also been used in the past to promote growth in food producing animals such as cattle and poultry.
The sulphonamides are competitive inhibitors of p-aminobenzoic acid in the folic acid metabolism cycle and have a bacteriostatic effect on a broad range of gram-positive and gram-negative organisms. The sulphonamides are distributed widely throughout all organs and tissues; and are metabolized primarily by the liver, producing inactive acetylated and glucuronide forms, which are excreted in urine.
Sulphonamides are used extensively in veterinary medicine; their use in food producing animals could result in potentially harmful concentrations in tissue, organs and milk. The potential risk is reduced by withdrawal of the drug for a fixed period before slaughter, although residual levels may still remain.
Regulatory authorities have specified MRLs (Maximum Residue Limits) for sulphonamide drugs, which are considered to represent safe levels for human consumption. The European Commission has specified a MRL of 100 ppb for all substances belonging to the sulphonamide group.
Principle
Antibody to SMT is bound to a microtitre plate. SMT (antigen) in the sample competes with the horseradish peroxidase labelled SMT (enzyme-labelled antigen) for binding to the limited number of antibody sites on the microtitre plate. The enzyme substrate is added. After an appropriate time has elapsed for maximum colour development, the enzyme reaction is stopped by the addition of acid, producing a colour change from blue to yellow. The optical density is read at 450 nm with the colour intensity being inversely proportional to the concentration of SMT in the sample.
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