Von willebrand factor ELISA
| Product | Method | Size | Catalog | Price | Quantity |
| Von willebrand factor ELISA | ELISA | 96T | VW3457 | £1043.00 | |
| Shipping costs will be added at the checkout stage, click here for charges. | |||||
Intended Use
For the quantitative in vitro determination of von Willebrand Factor (vWF) in plasma, serum or CSF.
General Background
Von Willebrand factor, a glycoprotein produced primarily by endothelial cells is also synthesised by megakaryocytes. If not secreted, the vWF is stored in intracellular organelles known as Weibel-Palade bodies, or α-granules. It is a multimeric protein composed of subunits that associate to form a wide range of high molecular weight multimers. The molecular weight of the multimers is thought to range from 500 to 20000 kDa.
Plasma vWF mediates platelet adhesion to damaged arterial walls and to other platelets, and also by stabilizing factor VIII. The most common inherited bleeding disorder is thought to be von Willebrand disease, with measurement of vWF being used as a diagnostic tool. In von Willebrand disease the levels of vWF are lower than normal. There have been a number of reports describing different associations of vWF with stroke. It has been found that vWF was a potent and independent risk factor for transient ischemic attack and minor ischemic stroke, with another study finding that there was no association with vWF levels and the development of first ever stroke. It has further been reported that the development of cerebral vasospasm after subarachnoid haemorrhage was preceded by an increase in serum vWF.
This ELISA aims to give a fast, reliable, analytical method to detect the presence of vWF. If performing the test using duplicate wells, this kit has the ability to analyse 40 samples.
Principle
A micro titre plate is supplied precoated with vWF antibody. vWF (antigen), if present in the sample binds to the vWF antibody. After incubation at 37oC the microtitre plate is washed to remove unbound antigen.
Horseradish peroxidase labelled vWF antibody (enzyme labelled antibody) is then added which binds to another epitope on the antigen. After incubation at 37oC the microtitre plate is washed to remove excess reagents.
The enzyme substrate is added. After an incubation period to allow maximum colour development, the colour reaction is stopped by addition of acid. This produces a colour change from blue to yellow, and the absorbances are read at 450 nm.
A calibration curve is then constructed to determine the vWF concentration in the sample.
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