Zeranol ELISA
| Product | Method | Size | Catalog | Price | Quantity |
| Zeranol ELISA | ELISA | 96T | ZR2421 | £1147.00 | |
| Shipping costs will be added at the checkout stage, click here for charges. | |||||
- Material Safety Data Sheets
Intended Use
For the quantitative in vitro determination of Zeranol in urine, muscle, liver and kidney samples. This product is suitable for Manual use.
General Background
Zeranol is a synthetic non-steroidal anabolic agent, with oestrogenic activity. The main metabolites of zeranol in mammals are zeralanone and taleranol. Zeranol and its metabolites are excreted as free compounds and as glucuronide or sulphate tracer. Zeranol is also produced naturally from mycotoxin zearalenone which is found in several strains of Fusaria that have been identified in feedstuffs. Therefore any tissue residues of zeranol may be of natural origin.
The regulatory approach to zeranol residues is varied. The joint FAO/WHO expert committee on food additives has recommended maximum residue limits (MRL) of 10ppb for zeranol in bovine liver and 2 ppb for zeranol in bovine muscle. The USA does not consider a tolerance for zeranol to be necessary. The European Union has provisionally prohibited the use of oestrogenic substances such as zeranol in food producing animals meaning that any detection of zeranol would be a violation. The European Union is currently working towards specifying MRPLs for assays for prohibited substances.
Principle
A micro titre plate is supplied precoated, with a zeranol antibody. Zeranol (antigen), if present in a standard or sample competes with horseradish peroxidase labelled zeranol (enzyme labelled antigen) for a limited number of antibody sites on the microtitre plate. After an incubation period at room temperature to allow a competition reaction to take place, the microtitre plate is then washed to remove excess reagents.
The enzyme substrate and chromogen are added and after an incubation period to allow maximum colour development, the colour reaction is stopped by the addition of Stop Solution. This produces a colour change from blue to yellow, and the absorbances are read at 450 nm.
A standard curve is then constructed to determine the zeranol concentration in the sample.
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