Home Clinical Chemistry Microalbumin (2-shot only) (liquid)

Microalbumin (2-shot only) (liquid)

Catalog # Name Size Price Qty  
MA2426 Microalbumin (2-shot only) (liquid)
Method: Immunoturbidimetric
R1 1 x 60ml, R2 3 x 8ml £172.86

Specification

Format: Liquid ready to use
Assay Range : 4.49 - 198mg/l
Working Stability 15-25 °C: _
Working Stability 2-8 °C: Stable to expiry
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Intended Use

For the quantitative in vitro determination of Microalbumin in urine. This product is suitable for both manual and automated use.


Clinical Significance


Kidney function may be assessed by measuring albumin levels in the urine. Normal kidney function entails filtering of waste products from the blood across tiny capillaries in the glomerulus.

Kidney malfunction results when the capillaries become blocked, causing waste products to remain in the blood and important proteins are lost from the blood into the urine.

Kidney deterioration is progressive and begins with small amounts of albumin leaking into the urine. This is known as microalbuminuria and indicates early signs of nephropathy. The term ‘micro-’ refers to low concentrations of urinary albumin. Progression of kidney disease will lead to larger amounts of albumin leaking into the urine which may develop further to end stage renal disease. Kidney disease is a major concern in diabetic patients and early detection and treatment may slow the onset and progression of the condition.

 

Condition     Albumin Levels (mg/day)
Normal    2-20
Microalbuminuria   20-300
Macroalbuminuria >300



Albumin is one of the major plasma proteins. In normal circumstances, albumin molecules are too large to cross the glomerular basement membrane; therefore, albumin is usually present at very low concentrations in the urine. Damage to the glomerular basement membrane can alter its permeability allowing albumin to enter the urine. Sustained elevation of urinary albumin concentrations is commonly referred to as Microalbuminuria.


Principle


Undiluted sample is added to a buffer containing antibody specific for human serum albumin. The absorbance (340 nm) of the resulting turbid solution is proportional to the concentration of albumin in the sample urine. By constructing a standard curve from the absorbances of standards, the albumin concentration of sample can be determined. The assay can be carried out manually (at room temperature) or using an automated analyser.