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Aldolase assay

Product Method Size Catalog Price Quantity
Aldolase assay UV 5 x 20ml AD189 $298.65
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  • Format
    Lyophilized
  • Assay Range
    1.73 - 106U/L
  • Working Stability 15-25 °C
    _
  • Working Stability 2-8 °C
    2 weeks
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Intended Use

For the quantitative in vitro determination of Aldolase in serum or plasma. This product is suitable for both manual and automated use.


Clinical Significance

Aldolase is an enzyme found throughout out the body however is present at high levels in muscle tissue. In humans the normal range is between 1 – 7.6 U/L, elevated levels can be detected in the blood of individuals with skeletal muscle damage, muscular dystrophy, cancer (liver, pancreatic or prostate) and liver disease making it a useful marker when diagnosing and monitoring the progression of muscle and liver diseases.

  • Skeletal muscle diseases – the skeletal muscles are attached to the bones and are responsible for those bones being able to move. In skeletal muscle diseases such as muscular dystrophy the cells become damaged and lyse as a result, releasing Aldolase into the bloodstream. The concentration of Aldolase in the blood can be directly related to the extent of muscle damage. Aldolase levels are also increased in non disease conditions affecting the muscle such as injury and gangrene however remain normal in cases where muscle weakness is caused by a neurological disease e.g. multiple sclerosis. The measurement of Aldolase can therefore help determine the root cause of muscle weakness either neurologic or muscular.
  • Liver disease – Although Aldolase is mainly found in skeletal muscle it is also present in the liver, damage to the liver by diseases such as chronic hepatitis also lead to an increase in Aldolase levels in the blood although to a lesser extent than skeletal muscle disease. Aldolase was used more so in the past to diagnose and monitor liver disease however it has largely been replaced by other enzymatic tests such as CK, ALT and AST.



Principle


Aldolase converts fructose­1,6­diphosphate (F­1,6­DP) to glyceraldehyde­3­phosphate (GAP) and dihydroxyacetone phosphate (DAP). The addition of triosephosphate isomerase (TIM), glycerolphosphate dehydrogenase (GDH) and NADH converts the dihydroxyacetone phosphate to glycerol­1­phosphate. The rate of the aldolase reaction is measured by the decrease in absorbance at 340 nm as a consequence of the conversion of NADH to NAD+.