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Alk. phos. assay

Product Method Size Catalog Price Quantity
Alk. phos. assay AMP (DIMENSION®) 360T AP2809 $194.72
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Intended Use

For the quantitative in vitro determination of Alkaline Phosphatase in serum or plasma. This product is suitable for use on the Siemens Dimension analyzers.


Clinical Significance

Serum Alkaline Phosphatase (ALP) measurements are of diagnostic use in the investigation and treatment of hepatobiliary disease such as hepatitis. In liver disease damaged liver cells release high levels of ALP into the blood. ALP levels are usually much lower than both AST and ALT however when bile ducts become blocked due to gallstones etc ALP levels are much higher than both AST and ALT.

ALP levels are also elevated in any condition that affects bone growth and in bone disease associated with increased osteoblastic activity. ALP can be used to help detect certain types of cancer that may have spread to the bone and bone conditions such as Paget’s disease where the boned become deformed. Also useful in monitoring cancer treatment.

ALP levels are also useful in the diagnosis of parathyroid and intestinal diseases.


Principle

The alkaline phosphatase method is based on a procedure published by Bowers and McComb and more recently reviewed by Rej. This method responds to all ALP isoenzymes in human serum.

Alkaline phosphatase catalyzes the transphosphorylation of p-nitrophenylphosphate (p-NPP) to p-nitrophenol (p-NP) in the presence of the transphosphorylating buffer, 2-amino-2-methyl-1-propanol (AMP). The reaction is enhanced through the use of magnesium and zinc ions. The change in absorbance at 405 nm due to the formation of p-NP is directly proportional to the ALP activity, since other reactants are present in non-rate limiting quantities and is measured using a bichromatic (405, 510 nm) rate technique.