Home Reagents Clinical Chemistry Reagents Alpha-1 antitrypsin assay
Quality Guranteed Fast Delivery ad for support

Alpha-1 antitrypsin assay

Product Method Size Catalog Price Quantity
Alpha-1 antitrypsin assay Immunoturbidimetric R1 3x16, R2 3x5ml AA2471 $761.00
Shipping costs will be added at the checkout stage, click here for charges.
  • Format
    Liquid ready to use
  • Assay Range
    40 - 640mg/dl
  • Working Stability 15-25 °C
    _
  • Working Stability 2-8 °C
    Stable to expiry
Request Kit Insert

Intended Use

Immunoturbidimetric assay for the quantitative in vitro determination of alpha-1-antitrypsin in human serum and plasma. This product is suitable for use on a wide range of automated analyzers.


Clinical Significance

Alpha-1-Antitrypsin is a glycoprotein made in the liver and plays a crucial role in protecting the lungs from damage caused by the enzyme neutrophil elastase. Emphysema is often the end result of such lung damage.

Alpha-1-Antitrypsin can therefore be used as a diagnostic marker to help diagnose emphysema especially in non smokers and individuals who have not been exposed to any lung irritants such as dust etc. The Alpha-1-Antitrypsin test can also used to determine a root cause for long term or persistent jaundice.

Alpha-1-Antitrpsin deficiency can be inherited with low levels in the blood (<80 mg/dl) indicating emphysema. Increased levels (3-4 times normal level) can occur as a result of trauma, pregnancy, administration of oestrogens or typhoid vaccine.


Principle

Patient samples are diluted and reacted with a buffer containing antibody specific for Alpha-1-Antitrypsin. The formation of the antibody-antigen complex during the reaction results in an increase in turbidity, the extent of which is measured as the amount of light absorbed at 340 nm. By constructing a standard curve from the absorbance of standards the Alpha-1-Antitrypsin concentration of a sample can be determined.