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Alt (gpt) (liquid) assay

Product Method Size Catalog Price Quantity
Alt (gpt) (liquid) assay IFCC R1 7 x 50ml, R2 2 x 44ml AL7904 $305.99
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  • Format
    Liquid ready to use
  • Assay Range
    4.25 - 400 U/L
  • Working Stability 15-25 °C
    _
  • Working Stability 2-8 °C
    4 weeks
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Intended Use

For the quantitative in vitro determination of Alanine Aminotransferase (ALT) in serum and plasma. This product is suitable for use on Hitachi 717, 911, and 912.


Clinical significance


The aminotransferases are a group of enzymes that catalyze the inter-conversion of amino acids and a oxoacids by transferring amino groups. The enzyme ALT (alanine aminotransferase or glutamate pyruvate transaminase) has been found to be in highest concentrations in the liver, with decreasing concentrations found in the kidneys, heart, skeletal muscle, pancreas, spleen and lung tissue respectively .

ALT measurements are used in the diagnosis and treatment of certain liver diseases (e.g. viral hepatitis and cirrhosis) and heart diseases. Extremely elevated levels of ALT can indicate acute hepatitis with moderately high levels indicating chronic hepatitis.

Elevated levels of the transaminases can also be indicative of myocardial infarction, hepatic disease, muscular dystrophy and organ damage. Elevated levels of ALT in serum is rare except in parenchymal liver disease, since ALT is a more liver specific enzyme.

ALT is often tested in combination with AST as part of a liver panel with ALT levels being higher in most types of liver disease.


Principle

This is a modification of the optimized standard method according to the recommendations of the IFCC.

α­oxoglutarate reacts with L­alanine in the presence of ALT to form L­glutamate plus pyruvate. The indicator reaction utilizes the pyruvate for a kinetic determination of NADH consumption.


Available Applications

  • Abbott Architect + Aeroset
  • Alcyon 300 + Falcor
  • BS-120, 200, 300 + 400
  • Dimension
  • Express 550
  • Hitachi 704,717, 902, 911, 912, 917
  • ILAB 600, 900 + 1800
  • Konelab 20i, 30i + 60i, Kone Progress
  • Olympus AU 400/600/ AU2700
  • Vitalab Flexor, Selectra E + Selectra II
  • Synchron CX4, 5, 7, 9 + LX20