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Alt (gpt) assay

Product Method Size Catalog Price Quantity
Alt (gpt) assay IFCC with P5P 240T AL2808 $160.81
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  • Format
    Mixing and diluting are automatically performed by the DIMENSIONĂ¢ instrument.
  • Assay Range
    53.5 - 1118U/L
  • Working Stability 15-25 °C
    _
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

For the quantitative in vitro determination of alanine aminotransferase activity in serum or plasma. This product is suitable for use on Siemens Dimension analyzers.


Clinical Significance

The aminotransferases are a group of enzymes that catalyze the inter-conversion of amino acids and a oxoacids by transferring amino groups. The enzyme ALT (alanine aminotransferase or glutamate pyruvate transaminase) has been found to be in highest concentrations in the liver, with decreasing concentrations found in the kidneys, heart, skeletal muscle, pancreas, spleen and lung tissue respectively .

ALT measurements are used in the diagnosis and treatment of certain liver diseases (e.g. viral hepatitis and cirrhosis) and heart diseases. Extremely elevated levels of ALT can indicate acute hepatitis with moderately high levels indicating chronic hepatitis.

Elevated levels of the transaminases can also be indicative of myocardial infarction, hepatic disease, muscular dystrophy and organ damage. Elevated levels of ALT in serum is rare except in parenchymal liver disease, since ALT is a more liver specific enzyme.

ALT is often tested in combination with AST as part of a liver panel with ALT levels being higher in most types of liver disease.


Principle

Alanine aminotransferase (ALT) catalyzes the transamination of L-alanine to α-ketoglutarate (α-KG), forming L-glutamate and pyruvate. The pyruvate formed is reduced to lactate by lactate dehydrogenase (LDH) with simultaneous oxidation of reduced nicotinamide-adenine dinucleotide (NADH). The change in absorbance is directly proportional to the ALT activity and is measured using a bichromatic (340, 700 nm) rate technique.