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Ammonia assay (manual)

Product Method Size Catalog Price Quantity
Ammonia assay (manual) Enzymatic UV 10 x 5ml AM1015 $158.33
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  • Format
    Lyophilised
  • Assay Range
    Linear to 1180 µmol/l
  • Working Stability 15-25 °C
    5 days
  • Working Stability 2-8 °C
    3 weeks
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Intended Use

For the quantitative in vitro determination of Ammonia in plasma. This product is suitable for Manual use.

Clinical Significance

The major source of circulating ammonia is the GI tract. Under normal conditions, ammonia is metabolised to urea by liver enzymes. Several diseases, both inherited and acquired, cause elevated ammonia (hyperammonemia). The inherited deficiencies of urea cycle enzymes are the major cause of hyperammonemia in infants. The acquired hyperammonemia diseases are caused by liver disease, renal failure and Reye’s Syndrome. Elevated ammonia is toxic to the central nervous system.

Principle

Ammonia combines with -ketoglutarate and NADPH in the presence of glutamate dehydrogenase (GLDH) to yield glutamate and NADP+. The corresponding decrease in absorbance at 340 nm is proportional to the plasma ammonia concentration.