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Ammonia assay

Product Method Size Catalog Price Quantity
Ammonia assay UV 10 x 5ml AM1015 $136.47
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  • Format
    Lyophilized
  • Assay Range
    Linear to 1180 µmol/l
  • Working Stability 15-25 °C
    5 days
  • Working Stability 2-8 °C
    3 weeks
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Intended Use

For the quantitative in vitro determination of Ammonia in plasma. This product is suitable for both automated and manual use.


Clinical Significance

The major source of circulating ammonia is the GI tract. Under normal conditions, ammonia is metabolized to urea by liver enzymes. Several diseases, both inherited and acquired can result in significantly elevated levels of ammonia in the blood (hyperammonemia).

Inherited deficiencies or defects of urea cycle enzymes is the major cause of hyperammonemia in infants however extremely high levels of ammonia may also be associated with hemolytic disease in newborn babies.

Reye’s syndrome an acquired hyperammonemia disease is characterized by high levels of ammonia and decreased levels of glucose as such ammonia is usually tested alongside glucose, electrolytes, kidney and liver function tests in adolescents and children showing symptoms.

High levels of ammonia in the blood are also associated with both liver disease and renal failure. Elevated ammonia is toxic to the central nervous system.


Principle


Ammonia combines with α­ketoglutarate and NADPH in the presence of glutamate dehydrogenase (GLDH) to yield glutamate and NADP+. The corresponding decrease in absorbance at 340 nm is proportional to the plasma ammonia concentration.


Available Applications

  • Various