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Methamphetamine assay

Product Method Size Catalog Price Quantity
Methamphetamine assay Homogeneous Enzyme Immunoassay R1 2 x 16.9ml, R2 2 x 8ml DA4007 $647.20
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  • Format
    Liquid ready-to-use
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

The Methamphetamine assay is an in vitro diagnostic test for the qualitative and semi-quantitative detection of methamphetamine in human urine on the RX daytona and RX imola analysers at the 1000 ng/ml cut-off. The assay is calibrated against methamphetamine.

This assay provides only a preliminary analytical result. A more specific alternative chemical must be used in order to obtain a confirmed analytical result. Gas Chromatography/Mass Spectrometry (GC/MS) is the preferred confirmatory. Clinical consideration and professional judgement should be exercised with any drug of abuse test result, particularly when the preliminary result is positive.

Clinical Significance

Methamphetamine is a synthetic derivative of ephedrine. It acts as a stimulant for the central nervous system, induces euphoria, alertness and a sense of increased energy. When Methamphetamine is ingested it is either rapidly deactivated in the liver or excreted unchanged into the urine.

Principle

The assay is based on competition between drug in the sample and drug labelled with the enzyme glucose-6-phosphate dehydrogenase (G6PDH) for a fixed amount of antibody in the reagent. Enzyme activity decreases upon binding to the antibody, and the drug concentration in the sample is measured in terms of enzyme activity.

In the absence of drug in the sample, methamphetamine-labelled G6PDH conjugate is bound to antibody, and the enzyme activity is inhibited. However, when free drug is present in the sample, antibody would bind to free drug; the unbound methamphetamine-labelled G6PDH then exhibits its maximal enzyme activity.

Active enzyme converts nicotinamide adenine dinucleotide (NAD) to NADH, resulting in an absorbance change that can be measured spectrophotometrically at 340 nm.

Available Applications

RX series instruments, including:

RX daytona

RX imola