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Methamphetamine assay

Product Method Size Catalog Price Quantity
Methamphetamine assay Homogenous EIA R1 2 x 16.9ml, R2 2 x 8ml DA4007 $647.20
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  • Format
    Liquid ready to use
  • Working Stability 15-25 °C
  • Working Stability 2-8 °C
    28 days
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Intended Use

For the qualitative and semi-quantitative analysis of Amphetamines in human urine. The cut-off for the qualitative application is 1000ng/ml as per SAMHSA recommendations. This product is suitable for use on the RX Series instruments, which includes the Rx Daytona and the Rx Imola.

This assay provides only a preliminary result. Clinical consideration and professional judgment should be applied to any drug of abuse test result, particularly in evaluating a preliminary positive result. To obtain a confirmed analytical result, a more specific alternate chemical method is needed. Gas chromatography/mass spectroscopy (GC/MS) is the recommended confirmatory method.

Clinical Significance

Amphetamines are a class of synthetic drugs with sympathomimetic activity i.e. they mimic the effects of the sympathetic nervous system. They cause powerful central nervous system stimulation and can result in euphoric effects similar to that of cocaine. Amphetamines can also alleviate fatigue, improve mental and physical performance and elevate mood, such effects have led to worldwide abuse of these legitimate drugs. Amphetamines are psychologically and physiologically addicting. Chronic, high dose abuse can lead to psychosis a condition indistinguishable with acute schizophrenia.

Amphetamines may also be used for a range of therapeutic applications for example; they can be used to treat mild depression, obesity, narcolepsy, and certain behavioral disorders in children.

The most common amphetamines include d-amphetamine, d-methamphetamine and d,l-amphetamine. Due to its ease of manufacture and ready availability, methamphetamine is the most abused of the amphetamines. Analogues of methamphetamine and amphetamine such as methylenedioxymethamphetamines (MDMA; Ecstasy) and 3, 4-methlendloxyamphetamine (MDA) have recently become popular at “rave parties” in both the United States and Europe.

Amphetamines can be taken orally, intravenously, or by smoking or snorting. They are rapidly absorbed from the gastrointestinal tract, and then either metabolized in the liver or excreted in urine unchanged. The presence of amphetamines may be detectable in urine for 3-4 days after administration.


The assay is based on competition between drug in the sample and drug labeled with the enzyme glucose-6-phosphate dehydrogenase (G6PDH) for a fixed amount of antibody in the reagent. Enzyme activity decreases upon binding to the antibody, and the drug concentration in the sample is measured in terms of enzyme activity.

In the absence of drug in the sample, amphetamines-labeled G6PDH conjugate is bound to antibody, and the enzyme activity is inhibited. However, when free drug is present in the sample, antibody would bind to free drug; the unbound amphetamines-labeled G6PDH then exhibits its maximal enzyme activity.

Active enzyme converts nicotinamide adenine dinucleotide (NAD) to NADH, resulting in an absorbance change that can be measured spectrophotometrically at 340 nm.