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Amylase (liquid) assay

Product Method Size Catalog Price Quantity
Amylase (liquid) assay EPS R1 6 x 20ml, R2 3 x 10ml AY7932 $660.68
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  • Format
    Liquid ready to use
  • Assay Range
    7.3 - 1509 U/L
  • Working Stability 15-25 °C
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

For the quantitative in vitro determination of a-amylase in serum and urine. This product is suitable for use on the Hitachi 704, 717, 902, 911 and 912.

Clinical Significance

The amylases are a group of hydrolases that split complex carbohydrates constituted of ω-D-glucose units. The two recognized types of amylases are ß-amylase (e.g. plant and bacterial exoamylase) and human ω-amylases which can attack the ω-1, 4-linkage anywhere along a polyglucan chain. Human ω-amylase consists of two major isoenzymes, pancreatic and salivary, which are encoded by two different genes. Pancreatic amylase is synthesized only in pancreatic tissue by acinar cells, however the salivary amylase is found in numerous locations, such as salivary glands and female genital, pulmonary and malignant tissues.

Amylase measurements are used primarily for the diagnosis and treatment of pancreatitis (severe inflammation of the pancreas) as well as pancreatic diseases. Amylase levels rise quickly at the start of a pancreatitis attack and fall after around 2 days. Elevated levels may also be associated with cancer of the pancreas, a perforated ulcer, gallbladder disease, mumps or ectopic pregnancy.


The method uses ethylidene blocked p-nitrophenyl-maltoheptaoside as substrate. The indicator enzyme α-glucosidase, used to release the p-nitrophenol, is also employed in the method. The terminal glucose of the substrate is chemically blocked preventing cleav­age by the indicator enzymes.

Available Applications

  • Abbott Architect + Aeroset
  • Alcyon 300 + Falcor
  • BS-120, BS-200, BS-300, BS-400
  • ILAB 600, 900, 1800
  • Clinline, Lisa Series + Mascott Plus
  • Express 550
  • Hitachi 704, 717, 902, 911, 912
  • Konelab 20i, 30i + 60i, Kone Progress
  • Olympus AU 400/560/600/ AU2700
  • Vitalab Flexor, Selectra E + Selectra II