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Amylase assay

Product Method Size Catalog Price Quantity
Amylase assay CNPG3 240T AY2811 $556.79
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  • Format
    Liquid ready to use
  • Assay Range
    10.4 - 1500U/L
  • Working Stability 15-25 °C
    _
  • Working Stability 2-8 °C
    4 weeks
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Intended Use

For the quantitative in vitro determination of Amylase in serum, plasma or urine. This product is suitable for use on the Siemens Dimension analyzers.


Clinical Significance

The amylases are a group of hydrolases that split complex carbohydrates constituted of ω-D-glucose units. The two recognized types of amylases are ß-amylase (e.g. plant and bacterial exoamylase) and human ω-amylases which can attack the ω-1, 4-linkage anywhere along a polyglucan chain. Human ω-amylase consists of two major isoenzymes, pancreatic and salivary, which are encoded by two different genes. Pancreatic amylase is synthesized only in pancreatic tissue by acinar cells, however the salivary amylase is found in numerous locations, such as salivary glands and female genital, pulmonary and malignant tissues.

Amylase measurements are used primarily for the diagnosis and treatment of pancreatitis (severe inflammation of the pancreas) as well as pancreatic diseases. Amylase levels rise quickly at the start of a pancreatitis attack and fall after around 2 days. Elevated levels may also be associated with cancer of the pancreas, a perforated ulcer, gallbladder disease, mumps or ectopic pregnancy.


Principle

α-amylase (α-1, 4-glucan, 4-glucanohydrolase; EC 3.2.1.1) catalyzes the hydrolysis of a defined synthetic substrate, 2-chloro-4-nitrophenyl-a-D-maltotrioside (CNPG3), to yield 2-chloro-4-nitrophenol (CNP), 2-chloro-4-nitrophenyl-a-D-maltoside (CNPG2), maltotriose (G3) and glucose. After an incubation of 70 seconds at 37ºC, the absorbance due to the formation of 2-chloro-4-nitrophenol (CNP) is measured using a bichromatic (405, 577 nm) rate technique.