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Apolipoprotein a-1 (liquid) assay

Product Method Size Catalog Price Quantity
Apolipoprotein a-1 (liquid) assay Immunoturbidimetric R1 4 x 30, R2 4 x 12ml LP3838 $663.32
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  • Format
    Liquid ready to use
  • Assay Range
    6.5 - 233mg/dl
  • Working Stability 15-25 °C
    _
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

For the quantitative in vitro determination of Apolipoprotein A-I (APO A-I) in serum and plasma. For use as an aid in assessing the risk of cardiovascular disease. This product is suitable for use on the RX Series instruments which includes the Rx Daytona and Rx Imola.


Clinical Significance

Lipids are metabolized in the intestine or liver, and are transported to tissues and organs after hydrophilic adaptation by a series of micellar structures. These structures consist of an outer monolayer of protein (an apolipoprotein) and polar lipids (phospholipids and unesterified cholesterol) plus an inner core of neutral lipids (triglycerides and cholesterol esters).

The apolipoproteins interact with a series of enzymes and tissue receptors and are therefore responsible for further metabolism and catabolism of the micelle.

Just as HDL and LDL are referred to as “good” and “bad” cholesterol, elevated levels of some apolipoproteins confer an increased cardiovascular risk while others have a protective effect. Apolipoproteins are a valuable addition to the lipid profile, supplying further information to researchers and clinicians. The genetic variants of the apolipoproteins can have a marked effect on cardiovascular risk and are linked with familial lipoprotein disorders such as hypertriglyceridaemia.

Apolipoproteins and the ratios between them are useful in the assessment of cardiovascular risk in individuals with normal lipoprotein levels. They are also valuable in specific patient populations, for example identifying patients that would benefit from more rigorous treatment in secondary prevention.

The A apolipoproteins are the main form of protein found in high density lipoproteins (HDL), although APO A-I is also present in chylomicrons. The main role of APO A-I is in the activation of Lecithin cholesterol acyl transferase (LCAT) and removal of free cholesterol from extra-hepatic tissues. APO A-I may therefore be described as non-atherogenic, showing an inverse relationship to cardiovascular risk.

Studies have shown that there is an inverse relationship between APO A-I and coronary artery disease and a direct relationship with APO B such that patients with CAD have generally reduced levels of APO A-I and increased levels of APO B.


Principle

This method is based on the reaction of a sample containing human APO A-I and specific antiserum to form an insoluble complex which can be measured turbidimetrically at 340 nm. By constructing a standard curve from the absorbances of standards the concentration of APO A-I can be determined.


Available Applications

  • RX Dedicated