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Apolipoprotein B (Apo B) assay

Product Method Size Catalog Price Quantity
Apolipoprotein B (Apo B) assay Immunoturbidimetric R1 4 x 50ml, R2 4.9ml LP2117 $1318.30
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  • Format
    Liquid ready to use
  • Assay Range
    11.5-219 mg/dl
  • Working Stability 15-25 °C
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

For the quantitative in vitro determination of apolipoprotein B (apo B) in serum and plasma. This product is suitable for use on Hitachi 704/717/902/911/912.

Clinical Significance

Lipids are synthesized in the intestine or liver but must be transported to tissues and organs, however this is not possible without hydrophilic adaptation. Lipids are therefore transported by a series of micellar structures. These structures consist of an outer monolayer of protein (an apolipoprotein) and polar lipids (phospholipids and unesterified cholesterol) plus an inner core of neutral lipids (tri­glycerides and cholestryl esters).

Just as HDL and LDL are referred to as “good” and “bad” cholesterol, elevated levels of some apolipoproteins confer an increased cardiovascular risk while others have a protective effect. Apolipoproteins are a valuable addition to the lipid profile, supplying further information to researchers and clinicians. The genetic variants of the apolipoproteins can have a marked effect on cardiovascular risk and are linked with familial lipoprotein disorders such as hypertriglyceridemia.

Apolipoproteins and the ratios between them are useful in the assessment of cardiovascular risk in individuals with normal lipoprotein levels. They are also valuable in specific patient populations, for example identifying patients that would benefit from more rigorous treatment in secondary prevention.

The B apolipoproteins are the main form of protein found in low density lipoproteins (LDL). Two forms of Apo B are found in humans. The most common form is B-100 (or large B) which constitutes the Apo B found in lipoproteins synthesized in the liver. The other form is B-48 (or small B), thought to be synthesized in the intestinal wall. Apo B is the main cholesterol carrying protein in the blood and is the ligand concerned with the uptake of cholesterol into cells by the LDL - receptor pathway. Apo B shows atherogenic signs and is thus useful for the evaluation of coronary risk.


This method is based on the reaction of a sample containing human apo B and a specific antiserum to form an insoluble complex which can be measured turbidimetrically at 340 nm. By constructing a standard curve from the absorbances of standards the concentration of apo B can be determined.