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B2 microglobulin assay

Product Method Size Catalog Price Quantity
B2 microglobulin assay Immunoturbidimetric R1 2 x 11ml, R2 2 x 4.3ml BM3887 $797.99
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  • Format
    Liquid ready to use
  • Assay Range
    0.56 - 20.9mg/l
  • Working Stability 15-25 °C
    _
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

Turbidimetric assay for the quantitative in vitro determination of b2Microglobulin in serum or urine. This product is suitable for use on the RX Series instruments which includes the Rx Daytona and Rx Imola.


Clinical Significance

Beta-2-Microglobulin (β2M) is a low molecular weight protein (11899D) found on the surface of most cells, including white blood cells. It is a constituent of the light chain of the human histocompatibility antigens HLA, A, B and C and may control the synthesis and expression of antigens on the cell surface. In healthy individuals, β2M is synthesized at a rate of 9mg/hour and is eliminated mostly by the kidneys.

When kidney disease is suspected, comparing blood and urine levels helps identify where the kidney is damaged. β2M is normally filtered out of the blood by the kidney’s glomeruli (a round mass of capillary loops leading to each kidney tubule), only to be partially reabsorbed back into the blood when it reaches the kidney’s tubules. In glomerular kidney disease, the glomeruli can’t filter it out of the blood, so levels increase in the blood and decrease in the urine. In tubular kidney disease, the tubules can’t reabsorb it back into the blood, so urine levels rise and blood levels fall. After a kidney transplant, increased blood levels may be an early sign of rejection.

Kidney damage may occur in individuals who have been exposed to high levels of heavy metals; high levels of β2M in urine may help detect the early stages of kidney damage.

In addition, patients on long-term hemodialysis, β2M can aggregate into amyloid fibers that deposit in joint spaces, causing a disease known as dialysis-related amyloidosis.

In AIDS, the serum level of β2M is an accurate indicator of the progression of the disease in HIV groups, including hemophiliacs. Levels above 5mg/L indicate rapid progression and poor prognosis. In various mylomas, β2M levels have been related to tumor mass, prognosis, disease activity and effectiveness of treatment. Levels below 4 mg/l indicate better prognosis than levels above 20 mg/l.


Principle

β2M present in patient samples reacts with the coated latex particles, causing an agglutination that can be measured at 546 nm. The increase in turbidity is proportional to the concentration of β2M in the sample.