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Benzodiazepine assay

Product Method Size Catalog Price Quantity
Benzodiazepine assay Homogeneous Enzyme Immunoassay R1 2 x 16.9ml , R2 2 x 8ml DA4009 $647.20
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  • Format
    Liquid ready-to-use
  • Assay Range
    _
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

The Benzodiazepine assay is an in vitro diagnostic test for the qualitative and semi-quantitative detection of Benzodiazepines in human urine on the RX imola and RX daytona analysers at the 200 ng/ml cut-off. The assay is calibrated against oxazepam.

This assay provides only a preliminary result. A more specific alternative chemical method must be used in order to obtain a confirmed analytical result. Gas Chromatography/ Mass Spectrometry (GC/MS) is the preferred confirmatory method. Clinical consideration and professional judgement should be exercised with any drug of abuse test result, particularly when the preliminary result is positive.

Clinical Significance

The benzodiazepine family has a diverse chemical structure which gives rise to a range of physio-chemical and pharmacological properties. In general, they are extensively metabolized by dealkylation, hydroxylation, oxidation and glucuronidation in the liver.

Principle

The assay is based on competition between drug in the sample and drug labelled with the enzyme glucose-6-phosphate dehydrogenase (G6PDH) for a fixed amount of antibody in the reagent. Enzyme activity decreases upon binding to the antibody, and the drug concentration in the sample is measured in terms of enzyme activity.

In the absence of drug in the sample, benzodiazepine-labelled G6PDH conjugate is bound to antibody, and the enzyme activity is inhibited. However, when free drug is present in the sample, antibody would bind to free drug; the unbound benzodiazepine-labelled G6PDH then exhibits its maximal enzyme activity.

Active enzyme converts nicotinamide adenine dinucleotide (NAD) to NADH, resulting in an absorbance change that can be measured spectrophotometrically at 340 nm.

Available Applications

RX series instruments, including:

RX daytona

RX imola