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Bilirubin (Direct) (liquid) assay

Product Method Size Catalog Price Quantity
Bilirubin (Direct) (liquid) assay Vanadate Oxidation R1 4 x 14ml, R2 4 x 6ml BR9765 $154.56
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  • Format
    Liquid ready-to-use
  • Assay Range
    0.95 - 262μmol/l (0.056 - 35.9mg/dl)
  • Working Stability 15-25 °C
    Stable to expiry date
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Intended Use

For the quantitative in vitro determination of Direct Bilirubin in serum and plasma. This product is suitable for use on the Hitachi 917 and Hitachi Modular P analyser.

Clinical Significance

Bilirubin is formed by the breakdown of haemoglobin in the spleen, liver and bone marrow. In the liver, bilirubin is conjugated with glucuronic acid to form a soluble compound. This conjugated bilirubin passes down the bile duct and is excreted into the gastrointestinal tract. An unconjugated, albumin bound form is also present in the circulation. It is insoluble and does not normally pass through the kidneys into the urine.

An increase in bilirubin concentration in the serum or tissues is called jaundice. Jaundice occurs in toxic or infectious diseases of the liver e.g. hepatitis B or obstruction of the bile duct and in rhesus incompatible babies.

Useful information may be obtained by determining which form of bilirubin is elevated.

High levels of conjugated or direct bilirubin indicate that bile is not being properly excreted; therefore an obstruction may be present in the bile duct or gall bladder. Unconjugated or indirect bilirubin can also be determined by subtracting the direct bilirubin level from the total bilirubin result. High levels of unconjugated bilirubin indicate that too much haemoglobin is being destroyed or that the liver is not actively treating the haemoglobin it is receiving.

Principle

The bilirubin is oxidized by vanadate at about pH 3 to produce biliverdin. In the presence of detergent and vanadate, conjugate (direct) bilirubin is oxidised. This oxidation reaction causes a decrease in the optical density of the yellow colour, which is specific to bilirubin. The decrease in optical density at 450/546 nm is proportional to the direct bilirubin concentration in the sample. The concentration is measured as an endpoint reaction.

Available Applications

Hitachi 917

Hitachi Modular P