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Bilirubin (liquid) assay

Product Method Size Catalog Price Quantity
Bilirubin (liquid) assay Jendrassik 1 x 225 or 2 x 625ml BR411 $46.50
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  • Format
    Liquid ready to use
  • Assay Range
    Total Bilirubin 1.83 - 560µmol/l, Direct Bilirubin 0.872 - 608µmol/l
  • Working Stability 15-25 °C
    Stable to expiry
  • Working Stability 2-8 °C
    _
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Intended Use

For the quantitative in vitro determination of total and direct bilirubin in serum or plasma. This product is suitable for both automated and manual use.


Clinical Significance

Bilirubin is formed by the breakdown of hemoglobin in the spleen, liver and bone marrow. In the liver, bilirubin is conjugated with glucuronic acid to form a soluble compound. This conjugated bilirubin passes down the bile duct and is excreted into the gastrointestinal tract. An unconjugated, albumin bound form is also present in the circulation. It is insoluble and does not normally pass through the kidneys into the urine.

An increase in bilirubin concentration in the serum or tissues can lead to a condition called jaundice. Jaundice occurs in toxic/infectious diseases of the liver e.g. hepatitis B, obstruction of the bile duct and in rhesus incompatible babies.

Too much bilirubin in the blood can indicate that the red blood cells are being destroyed too quickly or that the liver can not remove all of the bilirubin from the blood. In newborn babies excess bilirubin can lead to retardation or physical abnormalities, early detection is therefore extremely important.

Useful information may be obtained by determining which form of bilirubin is elevated. High levels of conjugated or direct bilirubin indicate that bile is not being properly excreted; therefore an obstruction may be present in the bile duct or gall bladder. Unconjugated or indirect bilirubin can also be determined by subtracting the direct bilirubin level from the total bilirubin result. High levels of unconjugated bilirubin indicate that too much hemoglobin is being destroyed or that the liver is not actively treating the hemoglobin it is receiving.


Principle

Colorimetric method based on that described by Jendrassik and Grof (1938). Direct (conjugated) bilirubin reacts with diazotised sulphanilic acid in alkaline medium to form a blue colored complex. Total bilirubin is determined in the presence of caffeine, which releases albumin bound bilirubin, by the reaction with diazotised sulphanilic acid.


Available Applications

  • Abbott Architect + Aeroset
  • Alcyon 300 + Falcor
  • Cobas Mira, Mira S + Mira Plus
  • BS 200, BS-300, BS-400
  • BT2000/BT3000/ILAB300/TARGA
  • Express 550
  • Hitachi 704, 717, 747, 904, 911,912
  • ILAB 300plus, 600
  • Konelab 20i, 30i + 60i
  • Olympus AU 400/560/600/ AU2700
  • Photometer 402
  • RA1000/XT/Opera
  • Synchron CX 4, CX 5, CX 7, CX 9, LX20
  • Vitalab Flexor, Selectra E + Selectra II
  • Advia 2400