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Bilirubin (total) assay

Product Method Size Catalog Price Quantity
Bilirubin (total) assay Modified Jendrassik 480T BR2802 $304.26
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  • Format
    Mixing and diluting are automatically performed by the DIMENSIONâ instrument.
  • Assay Range
    1.13 - 587µmol/l
  • Working Stability 15-25 °C
    _
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

The TBIL method used on the Dimension® clinical chemistry system is an in vitro diagnostic test intended for the quantitative determination of total bilirubin in serum and plasma. This product is suitable for use on the Siemens Dimension® analyzers.


Clinical Significance

Bilirubin is formed by the breakdown of hemoglobin in the spleen, liver and bone marrow. In the liver, bilirubin is conjugated with glucuronic acid to form a soluble compound. This conjugated bilirubin passes down the bile duct and is excreted into the gastrointestinal tract. An unconjugated, albumin bound form is also present in the circulation. It is insoluble and does not normally pass through the kidneys into the urine.

An increase in bilirubin concentration in the serum or tissues can lead to a condition called jaundice. Jaundice occurs in toxic/infectious diseases of the liver e.g. hepatitis B, obstruction of the bile duct and in rhesus incompatible babies.

Too much bilirubin in the blood can indicate that the red blood cells are being destroyed too quickly or that the liver can not remove all of the bilirubin from the blood. In newborn babies excess bilirubin can lead to retardation or physical abnormalities, early detection is therefore extremely important.

Useful information may be obtained by determining which form of bilirubin is elevated. High levels of conjugated or direct bilirubin indicate that bile is not being properly excreted; therefore an obstruction may be present in the bile duct or gall bladder. Unconjugated or indirect bilirubin can also be determined by subtracting the direct bilirubin level from the total bilirubin result. High levels of unconjugated bilirubin indicate that too much hemoglobin is being destroyed or that the liver is not actively treating the hemoglobin it is receiving.


Principle

The total bilirubin method is a modification of the Doumas reference method. The use of a caffeine/benzoate mixture to solubilise the protein bound un conjugated bilirubin was first reported by Enriques and Silvo. This method is a modification of the Jendrassik and Grof procedure.