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Cholesterol (liquid) assay

Product Method Size Catalog Price Quantity
Cholesterol (liquid) assay CHOD-PAP 6 x 30 CH200 $120.83
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  • Format
    Liquid ready to use
  • Assay Range
    13.7 - 656 ,g/dl
  • Working Stability 15-25 °C
    Stable to expiry
  • Working Stability 2-8 °C
    _
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Intended Use

For the quantitative in vitro determination of Cholesterol in serum and plasma. This product is suitable for manual for both manual and automated use.


Clinical Significance

Cholesterol measurements are used in the diagnosis and treatment of lipid and lipoprotein metabolism disorders. Lipids play an important role in the body; they serve as hormones or hormone precursors, aid in digestion, provide energy, storage and metabolic fuels, act as functional and structural components in biomembranes and form insulation to allow nerve conduction and prevent heat loss.

In clinical chemistry, over the last decade lipids have become associated with lipoprotein metabolism and atherosclerosis (hardening of the arteries). Elevated levels of cholesterol in the blood have been associated with atherosclerosis, heart disease and an increased risk of death from heart attack.

The American National Cholesterol Education Program (NCEP) Adult Treatment Panel (ATP) III recommends the following cut-off values for cholesterol:

  • Desirable – cholesterol levels below 200mg/dl (5.1 mmol/l) are considered desirable and indicate a low risk of heart disease
  • Borderline High – Levels between 200-239 mg/dl (5.18-6.18 mmol/L) generally reflect moderate risk of developing heart disease.
  • High – Cholesterol levels higher than 240 mg/dl (6.22 mmol/l) reflect high risk of developing heart disease.

Total cholesterol refers to cholesterol in all lipoprotein sub-classes and is measured to assess a patient's over all cholesterol status. Because it includes both 'good' HDL and 'bad' LDL, total cholesterol measurements alone cannot accurately predict CVD risk. Total cholesterol is useful as an initial screen but elevated levels would suggest a lipid profile is required.


Principle

The cholesterol is determined after enzymatic hydrolysis and oxidation. The indicator quinoneimine is formed from hydrogen peroxide and 4-aminoantipyrine in the presence of phenol and peroxidase.


Available Applications

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