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Cholesterol (Total) (liquid) assay

Product Method Size Catalog Price Quantity
Cholesterol (Total) (liquid) assay CHOD-PAP 6 x 100ml CH201 $379.95
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  • Format
    Liquid ready-to-use
  • Assay Range
    13.7 - 656 mg/dl (0.354 - 17.0mmol/l)
  • Working Stability 2-8 °C
    Stable to expiry when unopened and protected from light
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Intended Use

For the quantitative in vitro determination of Cholesterol in serum and plasma. This product is suitable for manual use and on the RX monza analyser.

Clinical Significance

Cholesterol measurements are used in the diagnosis and treatments of lipid lipoprotein metabolism disorders. Lipids play an important role in the body; they serve as hormones or hormone precursors, aid in digestion, provide energy, storage and metabolic fuels, act as functional and structural components in biomembranes and form insulation to allow nerve conduction and prevent heat loss.

In clinical chemistry, over the last decade however, lipids have become associated with lipoprotein metabolism and atherosclerosis. The Abell Kendell method, reported by Abell et al (1952) involved extraction of cholesterol by organic solvents and subsequent alkaline hydrolysis of the cholesterol esters. This reaction is highly specific but the reagents involved are corrosive and the method cumbersome, rendering it impractical for routine laboratory use.

The use of cholesterol oxidase following specimen saponification as described by Richmond (1973) provided the first step toward a totally enzymatic procedure. In 1974 Allain et al. and Roeschlaw et al. published the first fully enzymatic procedure for cholesterol determinations replacing chemical saponification with enzymatic saponification.

Principle

The cholesterol is determined after enzymatic hydrolysis and oxidation. The indicator quinoneimine is formed from hydrogen peroxide and 4-aminoantipyrine in the presence of phenol and peroxidase.

Available Applications

RX monza