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Cholesterol assay

Product Method Size Catalog Price Quantity
Cholesterol assay CHOD-PAP 480 Tests CH2823 $206.91
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  • Format
    Liquid ready to use
  • Assay Range
    0.754 - 20mmol/l
  • Working Stability 15-25 °C
    _
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

For the quantitative determination of cholesterol in serum and plasma. This product is suitable for use on the Siemens Dimension® analyzers.


Clinical Significance


Cholesterol measurements are used in the diagnosis and treatment of lipid and lipoprotein metabolism disorders. Lipids play an important role in the body; they serve as hormones or hormone precursors, aid in digestion, provide energy, storage and metabolic fuels, act as functional and structural components in biomembranes and form insulation to allow nerve conduction and prevent heat loss.

In clinical chemistry, over the last decade lipids have become associated with lipoprotein metabolism and atherosclerosis (hardening of the arteries). Elevated levels of cholesterol in the blood have been associated with atherosclerosis, heart disease and an increased risk of death from heart attack.

The American National Cholesterol Education Program (NCEP) Adult Treatment Panel (ATP) III recommends the following cut-off values for cholesterol:

  • Desirable – cholesterol levels below 200mg/dl (5.1 mmol/l) are considered desirable and indicate a low risk of heart disease
  • Borderline High – Levels between 200-239 mg/dl (5.18-6.18 mmol/L) generally reflect moderate risk of developing heart disease.
  • High – Cholesterol levels higher than 240 mg/dl (6.22 mmol/l) reflect high risk of developing heart disease

Total cholesterol refers to cholesterol in all lipoprotein sub-classes and is measured to assess a patient's over all cholesterol status. Because it includes both 'good' HDL and 'bad' LDL, total cholesterol measurements alone cannot accurately predict CVD risk. Total cholesterol is useful as an initial screen but elevated levels would suggest a lipid profile is required.


Principle

Cholesterol esterase (CE) catalyzes the hydrolysis of cholesterol esters to produce free cholesterol which, along with pre-existing free cholesterol, is oxidized in a reaction catalyzed by cholesterol oxidase (CO) to form cholest-4-ene-3-one and hydrogen peroxide. In the presence of horseradish peroxidase (HPO), the hydrogen peroxide thus formed is used to oxidize 4-aminoantipyrine to produce the indicator quinonimine that absorbs at 510 nm. The absorbance due to quinonimine indicator formed is directly proportional to the total cholesterol concentration and is measured using a bichromatic (510, 600 nm) endpoint technique.


Available Applications

  • RX daytona, RX imola
  • Cobas Mira, Mira S + Mira Plus
  • Dimension dedicated