Home Reagents Clinical Chemistry Reagents Ck-nac assay
Quality Guranteed Fast Delivery ad for support

Ck-nac assay

Product Method Size Catalog Price Quantity
Ck-nac assay SZASZ (DIMENSION®) 480T CK2813 $440.30
Shipping costs will be added at the checkout stage, click here for charges.
Request Kit Insert

Intended Use

The CK method used on the Dimension® clinical chemistry system is an in vitro diagnostic test intended for the quantitative determination of creatine kinase activity in serum and plasma. This product is suitable for use on the Siemens Dimension® analyzers.


Clinical Significance


Creatine Kinase (CK) is a dimeric enzyme that catalyzes the conversion of creatine to creatine phosphate, it is composed of two sub-units Muscle (M) and Brain (B) which associate to form three isoenzymes CK-MM, CK-MB and CK-BB. CK is present in many organs throughout the body but is perhaps found primarily in striated muscle, brain and heart tissues. Damage or disease to any of these tissues will result in elevated levels of the enzyme in the blood.

Determination of CK activity in both plasma and serum can therefore provide a sensitive and specific marker for myocardial infarction, hyperthyroidism, kidney disease, cerebrovascular accidents and skeletal muscle diseases such as muscular dystrophy.

The determination of CK using creatine phosphate and adenosine-5'-diphosphate (ADP) as substrates rather than creatine and adenosine-5'-triphosphate (ATP) has several advantages in test performance as it allows for a faster reaction rate resulting in greater sensitivity.


Principle

The determination of CK using creatine phosphate and adenosine-5'-diphosphate (ADP) as substrates rather than creatine and adenosine-5'-triphosphate (ATP) has several advantages in test performance as it allows for a faster reaction rate resulting in greater sensitivity. Small sample volumes are used and sample blanks are not required.

Due to the oxidation of the sulphydryl groups in the active site of creatine kinase, it is very unstable. The enzyme however, is reactivated by the additon of thiol compounds.

N-acetyl-L-cysteine (NAC), is included in the test for this reason. Also included in the test system are adenosine-5'- monophosphate (AMP) and diodenosine pentaphosphate, these are present to inhibit myokinase activity which interferes. No sulphates are present in the test system as these alter the reation kinetics.