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Cocaine Metabolite assay

Product Method Size Catalog Price Quantity
Cocaine Metabolite assay Homogeneous Enzyme Immunoassay R1 2 x 16.9ml, R2 2 x 8ml DA4011 $647.20
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  • Format
    Liquid ready-to-use
  • Assay Range
    _
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

The Cocaine assay is an in vitro diagnostic test for the qualitative and semi-quantitative detection of benzoylecgonine (Cocaine Metabolite) in human urine on the RX imola and RX daytona analysers at the 300 ng/ml cut-off. The assay is calibrated against Benzoylecgonine.

This assay provides only a preliminary result. A more specific alternative chemical method must be used in order to obtain a confirmed analytical result. Gas Chromatography/Mass Spectrometry (GC/MS) is the preferred confirmatory method. Clinical consideration and professional judgement should be exercised with any drug of abuse test result, particularly when the preliminary result is positive.

Clinical Significance

Cocaine (methylbenzoylecgonine) is an alkaloid found in the plant Erythroxylon coca, which is principally grown in South America. It is chemically, but not pharmacologically, related to atropine.

Cocaine is a CNS stimulant; however, it also exhibits numerous undesirable side effects including cardiac toxicity and behaviour responses such as paranoia and hallucinations. The most important action of cocaine clinically is its ability to block nerve conductance upon local application.

Cocaine sold on the street includes the hydrochloride salt and crack cocaine. The salt is frequently abused by inhalation or intravenous injection. Crack is a free base form of cocaine that produces a characteristic cracking sound when smoked. Cocaine is rapidly metabolised with less than 5% excreted unchanged in urine. The major metabolite is benzoylecgonine. Other notable metabolites are methylecgonine and ecgonine. Cocaine metabolites are detectable in urine for 1-3 days after moderate use. However for long term, heavy use, the metabolites may be found in urine for up to 3 weeks. Cocaine readily passes through the placenta into the foetus. Cocaine abuse during pregnancy can adversely affect the foetal development and cause serious problems in the neonate.

Principle

The assay is based on competition between drug in the sample and drug labelled with the enzyme glucose-6-phosphate dehydrogenase (G6PDH) for a fixed amount of antibody in the reagent. Enzyme activity decreases upon binding to the antibody, and the drug concentration in the sample is measured in terms of enzyme activity.

In the absence of drug in the sample, benzoylecgonine-labelled G6PDH conjugate is bound to antibody, and the enzyme activity is inhibited. On the other hand, when free drug is present in the sample, antibody would bind to free drug; the unbound benzoylecgonine-labelled G6PDH then exhibits its maximal enzyme activity.

Active enzyme converts nicotinamide adenine dinucleotide (NAD) to NADH, resulting in an absorbance change that can be measured spectrophotometrically at 340 nm.

Available Applications

RX series instruments, including:

RX daytona

RX imola