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Hdl-Cholesterol (manual) assay (Precipitant)

Product Method Size Catalog Price Quantity
Hdl-Cholesterol (manual) assay (Precipitant) Phosphotungstic Acid 4 x 80ml CH203 $168.18
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  • Format
    Liquid ready-to-use
  • Assay Range
    0.071 - 23.7mmol/l (2.75 - 915mg/dl)
  • Working Stability 15-25 °C
    Stable to expiry
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Intended Use

For the quantitative in vitro determination of HDL-Cholesterol in serum and plasma. This product is suitable for manual use and on the Rx Monza analyser.

Supplementary pack for Cholesterol, CHOD-PAP method.

Clinical Significance

High-density lipoproteins (HDL) are one of the major classes of plasma lipoproteins. They are composed of a number of heterogeneous particles, including cholesterol and vary with respect to size and content of lipid and apolipoprotein. HDL serve to remove cholesterol from the peripheral cells to the liver, where the cholesterol is converted to bile acids and excreted into the intestine.

An inverse relationship between HDL-cholesterol (HDL-C) levels in serum and the incidence/prevalence of coronary heart disease (CHD) has been demonstrated in a number of epidemiological studies. The importance of HDL-C as a risk factor for CHD is now recognised.

Accurate measurement of HDL-C is of vital importance when assessing patient risk from CHD. In this diagnostic test kit a method for direct measurement of HDL-C, without sample pretreatment, is presented. Direct measurement gives improved accuracy and reproducibility when compared to precipitation methods.

Principle

Low density lipoproteins (LDL and VLDL) and chylomicron fractions are precipitated quantitatively by the addition of phosphotungstic acid in the presence of magnesium ions. After centrifugation, the cholesterol concentration in the HDL (high density lipoprotein) fraction, which remains in the supernatant, is determined.

Available Applications

RX monza