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Hdl-cholesterol (liquid) assay

Product Method Size Catalog Price Quantity
Hdl-cholesterol (liquid) assay Clearance R1 6 x 30, R2 3x 20ml CH2652 $937.25
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  • Format
    Liquid ready to use
  • Assay Range
    Linear to 3.56mmol/l
  • Working Stability 15-25 °C
    _
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

Enzymatic Clearance assay for the in vitro quantitative determination of HDL-cholesterol in human serum and plasma. This product is suitable for use on Cobas Mira, Mira-S, Mira Plus, Hitachi 704, 717, 737, 747, 902, 904, 911, 912, 917, Modular P, Synchron CX®4/5/7/9 and Synchron LX®20.


Clinical Significance

High-density lipoproteins (HDL) are one of the major classes of plasma lipoproteins. They are composed of a number of heterogeneous particles, including cholesterol and vary with respect to size and content of lipid and apolipoprotein.

HDL serves to remove cholesterol from the peripheral cells to the liver, where the cholesterol is converted to bile acids and excreted into the intestine. HDL cholesterol is often referred to as ‘good cholesterol’, an inverse relationship between HDL cholesterol levels in the serum and the incidence/prevalence of coronary heart disease (CHD) has been demonstrated in a number of epidemiological studies. The importance of HDL cholesterol as a risk factor for CHD is now recognized.

HDL cholesterol is usually measured alongside total cholesterol, LDL cholesterol and triglycerides as part of a lipid profile. High levels of HDL cholesterol in the blood can lower an individual’s risk of developing heart disease, if the HDL cholesterol accounts for 20% of total cholesterol then the risk of developing heart disease is less than average similarly an HDL cholesterol result of 1.5mmol/L or more then the risk of developing heart disease is less than average.

Accurate measurement of HDL cholesterol is of vital importance when assessing patient risk from CHD. In this diagnostic test kit a method for direct measurement of HDL cholesterol, without sample pre-treatment, is presented. Direct measurement gives improved accuracy and reproducibility when compared to precipitation methods.


Principle


The assay consists of 2 distinct reaction steps:

  1. Elimination of chylomicron, VLDL-Cholesterol and LDL-Cholesterol by cholesterol esterase, cholesterol oxidase and subsequently catalase.
  2. Specific measurement of HDL-Cholesterol after release of HDL-Cholesterol by detergents in Reagent 2.

The intensity of the quinone imine dye produced is directly proportional to the cholesterol concentration when measured at 600 nm.


Available Applications

  • Abbott Architect + Aeroset + spectrum
  • Alcyon 300 + Falcor
  • ATAC 6000, 8000
  • Axon
  • BS-200, BS-300
  • BT2000/BT3000/ILAB300/TARGA
  • Chemwell
  • ILAB 1800, 900
  • Clinline, Lisa Series + Mascott Plus
  • Cobas 6000
  • Cobas Mira, Mira S + Mira Plus, Fara, Bio
  • Hitachi 704, 717, 737, 747, 902, 904, 911, 912, 917
  • Synchron CX 4, CX 5, CX 7, CX 9, LX20
  • Express 550
  • Humalyzer 850, Humalyzer 900S
  • ILAB 300plus, 600
  • Integra 400
  • Konelab 20i, 30i + 60i, progress, specific
  • Pentra 400
  • Prestige
  • RA1000, XT, Opera
  • Vitalab Flexor, Selectra E + Selectra II
  • Olympus AU2700, 400, 5000, 5200, 560, 600, 800,