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HDL-Cholesterol (Direct) assay

Product Method Size Catalog Price Quantity
HDL-Cholesterol (Direct) assay Direct Clearance R1 6 x 78ml, R2 3 x 52ml CH2655 $2081.31
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  • Format
    Liquid ready-to-use
  • Assay Range
    Cobas (0.251 - 3.92mmol/l), Hitachi (0.036 - 3.71mmol/l), Sychron (0.176 - 4.03mmol/l)
  • Working Stability 2-8 °C
    Stable to expiry when unopened and protected from light
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Intended Use

Enzymatic Clearance assay for the in vitro quantitative determination of HDL-cholesterol in human serum and plasma. This product is suitable for use on Cobas Mira, Mira-S, Mira Plus, Hitachi 704, 717, 737, 747, 902, 904, 911, 912, 917, Modular P, Synchron CX®4/5/7/9, LX®20 and UniCel DxC 600/800 analysers.

Clinical Significance

High-density lipoproteins (HDL) are one of the major classes of plasma lipoproteins. They are composed of a number of heterogeneous particles, including cholesterol and vary with respect to size and content of lipid and apolipoprotein. HDL serve to remove cholesterol from the peripheral cells to the liver, where the cholesterol is converted to bile acids and excreted into the intestine.

An inverse relationship between HDL-cholesterol (HDL-C) levels in serum and the incidence/prevalence of coronary heart disease (CHD) has been demonstrated in a number of epidemiological studies. The importance of HDL-C as a risk factor for CHD is now recognised.

Accurate measurement of HDL-C is of vital importance when assessing patient risk from CHD. In this diagnostic test kit a method for direct measurement of HDL-C, without sample pretreatment, is presented. Direct measurement gives improved accuracy and reproducibility when compared to precipitation methods.

Principle

The assay consists of 2 distinct reaction steps:

1. Elimination of chylomicron, VLDL-Cholesterol and LDL-Cholesterol by cholesterol esterase, cholesterol oxidase and subsequently catalase. Cholesterolesterase

2. Specific measurement of HDL-Cholesterol after release of HDL-Cholesterol by detergents in Reagent 2.

The intensity of the quinone imine dye produced is directly proportional to the cholesterol concentration when measured at 600 nm.

In the second reaction catalase is inhibited by sodium azide in Enzyme Reagent 2.

This assay uses a Rate method and a single point calibration.

Available Applications

Cobas Mira

Cobas Mira-s

Cobas Mira Plus

Hitachi 704

Hitachi 717

Hitachi 737

Hitachi 747

Hitachi 902

Hitachi 904

Hitachi 911

Hitachi 912

Hitachi 917

Hitachi Modular P

Synchron CX 4 / 5 / 7 / 9

Synchron LX20

Unicel DXC 600 / 800