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H-FABP (Heart-type fatty acid binding protein) assay

Product Method Size Catalog Price Quantity
H-FABP (Heart-type fatty acid binding protein) assay Immunoturbidimetric R1 1 x 19ml, R2 1 x 7ml FB4025 $1900.35
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  • Format
    Liquid ready-to-use
  • Assay Range
    0.747 - 120ng/ml
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

For the quantitative in vitro determination of H-FABP concentration in serum or plasma.

Clinical Significance

Heart–type Fatty Acid Binding Protein (H-FABP) is a low molecular-weight (15kD) cytoplasmic protein that is involved in the intracellular uptake and buffering of free fatty acids in the myocardium6. The combination of its low molecular weight and cytoplasmic location enables H-FABP to be a highly sensitive early rise marker of acute coronary syndrome, detectable as early as 30 minutes following the onset of an ischemic episode7. H-FABP concentrations peak at approximately 6-8 hours and return to normal within approximately 24-30 hours8. Although H-FABP has similar release kinetics to Myoglobin, it is approximately 15-20 times more cardiac specific, hence making it a more effective marker of myocardial injury.

Using a combination of H-FABP and Troponin has been shown to significantly improve the diagnostic sensitivity for MI/ACS during the early hours after symptom onset (at presentation,10 <4 hours11 or <6 hours12) compared to using Troponin alone. Prognostically, a number of large trials have illustrated the value of H-FABP in stratifying long-term ACS risk in both Troponin positive and Troponin negative patients13,14,15. H-FABP has also been shown to be incrementally additive to Troponin, diagnostically and prognostically, even when a highly sensitive Troponin assay is used.

In addition to ACS, H-FABP has been found to be clinically useful in a range of other applications, such as pulmonary embolism (PE)17, coronary artery bypass surgery (CABG)18 and cerebrovascular disease.

Principle

Sample is reacted with a buffer and anti-H-FABP coated latex. The formation of the antibody-antigen complex during the reaction results in an increase in turbidity, the extent of which is measured as the amount of light absorbed at 700 nm. By constructing a standard curve from the absorbance of the standards, H-FABP concentration in the sample can be determined.