Home Reagents Clinical Chemistry Reagents Hydroxybutyrate assay (RANBUT) assay
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Hydroxybutyrate assay (RANBUT) assay

Product Method Size Catalog Price Quantity
Hydroxybutyrate assay (RANBUT) assay Enzymatic 10 x 50ml RB1008 $890.16
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  • Format
    Lyophilized
  • Assay Range
    0.1 - 3.2mmol/l
  • Working Stability 15-25 °C
    24 hours
  • Working Stability 2-8 °C
    7 days
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Intended Use

For the quantitative in vitro determination of D-3-Hydroxybutyrate in serum and plasma. This product is suitable for manual use and on the Rx Monza.


Clinical Significance


The function of insulin is to enable the transport of glucose from the blood into the cells. When insulin levels are insufficient, glucose cannot be transported into the cells meaning fat is utilized for energy production. Fat is broken down into glycerin and free fatty acids, which can be used as a source of energy. Metabolism of fatty acids in the liver results in the production of ketone bodies, consisting of acetone (2%), acetoacetate (20%) and D-3 hydroxybutyrate (78%). Increased levels of ketones can lead to the very dangerous condition ketoacidosis and if untreated can eventually lead to diabetic coma.

The nitroprusside method of ketone detection used in semi-quantitative dipstick tests detects acetone and acetoacetate, but not D-3-hydroxybutyrate. Ranbut measures D-3-hydroxybutyrate, the major ketone body in the blood making it a very sensitive test.


Principle


A kinetic enzymatic method to measure the level of D-3-hydroxybutyrate in serum or plasma. The method is based on the oxidation of D-3-hydroxybutyrate to acetoacetate by the enzyme 3-Hydroxybutyrate dehydrogenase. Concomitant with this oxidation the cofactor NAD+ is reduced to NADH and the associated change of absorbance can be directly correlated with the D-3-hydroxybutyrate concentration.


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