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Iron assay

Product Method Size Catalog Price Quantity
Iron assay Ferrozine 4 x 60 tests SI2818 $316.87
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  • Format
    Mixing and diluting are automatically performed by the instrument.
  • Assay Range
    0.4 - 222umol/L
  • Working Stability 15-25 °C
  • Working Stability 2-8 °C
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Intended Use

The IRN method used on the Dimension® clinical chemistry system is an in vitro diagnostic test intended for the quantitative determination of iron in serum.

Clinical Significance

The concentration of serum iron reflects principally the concentration of ferric iron bound to serum transferrin, a protein responsible for transporting iron from the intestine to the cells were it is needed.

Iron deficiency is one of the most prevalent human disorders. In adults iron deficiency is often symptomatic of chronic blood loss. Iron measurements are particularly beneficial when determining if anemia is due to iron deficiency or long term illness. Serum iron concentrations also appear to be decreased in acute or chronic inflammatory disorders such as acute infection, immunization or myocardial infarction.

Elevated levels of serum iron are present in hemochromatosis a genetic condition where too much iron is present in the body. Higher than normal levels of iron are also known to occur as a result of blood transfusion, hepatic disease, renal disease and lead poisoning.


The iron method is an adaptation of direct iron assays developed using the chromophore Ferene.

Early work demonstrated the high sensitivity of Ferene® and its utility in iron assays. The IRN method is a direct iron procedure using a surfactant to prevent protein precipitation. A serum blank is used to correct for differences in specimen turbidity. Potential copper interference is minimized by the addition of thiourea. Ferene® is a registered trademark of Diagnostic Chemicals, LTD., Charlottetown, P.E.I., Canada C1A4H5.

Under acidic conditions (pH 4.5), iron bound to the protein transferrin is released in the presence of the reducing agent, ascorbic acid. The resulting product, Fe++, forms a blue complex with 3-(2-pyridyl)-5,6-bis-2-(5-furyl sulfonic acid)-1,2,4-triazine, disodium salt (Ferene). The absorbance of the complex, measured using a bichromatic (600, 700 nm) endpoint technique, is proportional to the concentration of transferrin-bound iron in the serum.

Available Applications

  • Dimension dedicated