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Ldh assay

Product Method Size Catalog Price Quantity
Ldh assay NAD 4 x 12o tests LD2812 $365.11
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  • Format
    Mixing and diluting are automatically performed by the instrument.
  • Assay Range
    6.7 - 566U/L
  • Working Stability 15-25 °C
    _
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

The LDH method used on the Dimension® clinical chemistry system is an in vitro diagnostic test intended for the quantitative determination of lactate dehydrogenase activity in serum or plasma. This product is suitable for use on the Siemens Dimension® analyzers.


Clinical Significance

Lactate dehydrogenase (LDH) catalyzes the interconversion of lactic acid and pyruvic acid. The enzyme is composed of 4 peptide chains and exists in 5 isomeric forms. LDH is widely distributed throughout the body; highest concentrations are found in the liver, heart and skeletal muscle.

LDH is a general marker of tissue damage and is often used to determine the root cause and location of damage.

LD activity is significantly elevated during myocardial infarction. Maximum levels are reached 24 to 48 hours after the on set of chest pain and may remain elevated for 7 to 12 days postinfarction.

Increased activity is also associated with stroke, kidney disease, liver disease, progressive muscular dystrophy, cancer, intestinal and pulmonary infarction.


Principle

The LDH method measures the oxidation of L-lactate to pyruvate with simultaneous reduction of nicotinamide adenine dinucleotide (NAD). The change in absorbance at 340 nm due to the appearance of reduced NAD (NADH) is directly proportional to the LDH activity, since other reactants are present in non-rate limiting quantities and is measured using a bichromatic (340, 383 nm) rate technique.


Available Applications

  • Dimension dedicated