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Ldl- cholesterol (liquid) assay

Product Method Size Catalog Price Quantity
Ldl- cholesterol (liquid) assay Clearance R1 3 x 51ml, R2 3 x 20ml CH3841 $1488.35
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  • Format
    Liquid ready to use
  • Assay Range
    0.189 - 22.2mmol/l
  • Working Stability 15-25 °C
    _
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

An LDL-cholesterol test system is a device intended for the quantitative in vitro determination of LDL-cholesterol concentration in human serum and plasma. This product is suitable for use on the RX Series instruments which includes the Rx Daytona and Rx Imola.


Clinical Significance

Low Density Lipoproteins (LDL) are synthesized in the liver by the action of various Lipolytic enzymes on triglyceride rich Very Low Density Lipoproteins (VLDLs).

LDL cholesterol is often referred to as ‘bad cholesterol’ since LDL transports cholesterol to the tissues and is linked to the development of artherosclerotic lesions. For this reason LDL cholesterol is considered to be the most important clinical predictor, of all single parameters, with respect to coronary atherosclerosis.

Risk factors for heart disease include age, family history, smoking, low HDL levels, diabetes and high blood pressure. If an individual has no other risk factors an LDL concentration of 160 – 189 mg/dl or 4.15 – 4.90 mmol/L is considered high.

Accurate measurement of LDL-Cholesterol is of vital importance in therapies which focus on lipid reduction to prevent atherosclerosis or reduce its progress and to avoid plaque rupture. In this diagnostic test kit an elimination method for the measurement of LDL-Cholesterol, without sample pre-treatment, is presented which correlates well with precipitation methods.


Principle

The assay consists of 2 distinct reaction steps:

  1. Elimination of chylomicron, VLDL-Cholesterol and HDL-Cholesterol by cholesterol esterase, cholesterol oxidase and subsequently catalase.
  2. Specific measurement of LDL-Cholesterol after release of LDL-Cholesterol by detergents in Reagent 2.

The intensity of the quinoneimine dye produced is directly proportional to the cholesterol concentration when measured at 600 nm.


Available Applications

  • RX dedicated