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LDL-Cholesterol (Direct) assay

Product Method Size Catalog Price Quantity
LDL-Cholesterol (Direct) assay Direct Clearance R1 3 x 51ml, R2 3 x 20ml CH3841 $1790.27
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  • Format
    Liquid ready-to-use
  • Assay Range
    0.189 - 22.2mmol/l (7.30 - 860mg/dl)
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

An LDL-cholesterol test system is a device intended for the quantitative in vitro determination of LDL-cholesterol concentration in human serum and plasma. This product is suitable for use on RX series instruments which includes the RX daytona and the RX imola.

Clinical Significance

Low Density Lipoproteins (LDL) are synthesised in the liver by the action of various Lipolytic enzymes on triglyceride rich Very Low Density Lipoproteins (VLDLs). Specific LDL receptors exist to facilitate the elimination of LDL from plasma by liver parenchymal cells. It has been shown that most of the cholesterol stored in atherosclerotic plaques originates from LDL. For this reason the LDL-Cholesterol concentration is considered to be the most important clinical predictor, of all single parameters, with respect to coronary atherosclerosis.

Accurate measurement of LDL-Cholesterol is of vital importance in therapies which focus on lipid reduction to prevent atherosclerosis or reduce its progress and to avoid plaque rupture.

In this diagnostic test kit an elimination method for the measurement of LDL-Cholesterol, without sample pretreatment, is presented which correlates well with precipitation methods.

Principle

The assay consists of 2 distinct reaction steps:

1. Elimination of chylomicron, VLDL-Cholesterol and HDLCholesterol by cholesterol esterase, cholesterol oxidase and subsequently catalase.

2. Specific measurement of LDL-Cholesterol after release of LDL-Cholesterol by detergents in Reagent 2.

The intensity of the quinoneimine dye produced is directly proportional to the cholesterol concentration when measured at 600 nm.

In the second reaction catalase is inhibited by sodium azide in Enzyme Reagent 2.

Available Applications

RX series instruments, including:

RX daytona

RX imola