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LDL-Cholesterol (Direct) assay

Product Method Size Catalog Price Quantity
LDL-Cholesterol (Direct) assay Direct Clearance R1 6 x 78ml, R2 3 x 52ml CH2656 $4611.22
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  • Format
    Liquid ready-to-use
  • Assay Range
    Cobas (0.440 - 25.3mmol/l), Hitachi (0.784 - 26.4mmol/l), Synchron (0.500 - 25.4mmol/l)
  • Working Stability 2-8 °C
    Stable to expiry when unopened and protected from light
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Intended Use

Enzymatic Clearance assay for the in vitro quantitative determination of LDL-cholesterol in human serum and plasma. This product is suitable for use on the Cobas Mira, Mira-S, Mira Plus, Hitachi 704, 717, 737, 747, 902, 904, 911, 912, 917, Modular P, Synchron CX® 4/5/7/9, LX® 20 and UniCel DxC 600/800 analysers.

Clinical Significance

Low Density Lipoproteins (LDL) are synthesised in the liver by the action of various Lipolytic enzymes on triglyceride rich Very Low Density Lipoproteins (VLDLs). Specific LDL receptors exist to facilitate the elimination of LDL from plasma by liver parenchymal cells. It has been shown that most of the cholesterol stored in atherosclerotic plaques originates from LDL. For this reason the LDL-Cholesterol concentration is considered to be the most important clinical predictor, of all single parameters, with respect to coronary atherosclerosis.

Accurate measurement of LDL-Cholesterol is of vital importance in therapies which focus on lipid reduction to prevent atherosclerosis or reduce its progress and to avoid plaque rupture.

In this diagnostic test kit, an elimination method for the measurement of LDL-Cholesterol, without sample pretreatment, is presented which correlates well with precipitation methods.

Principle

The assay consists of 2 distinct reaction steps:

1. Elimination of chylomicron, VLDL-Cholesterol and HDL-Cholesterol by cholesterol esterase, cholesterol oxidase and subsequently catalase.

2. Specific measurement of LDL-Cholesterol after release of LDL-Cholesterol by detergents in Reagent 2.

The intensity of the quinoneimine dye produced is directly proportional to the cholesterol concentration when measured at 600 nm.

In the second reaction catalase is inhibited by sodium azide in Enzyme Reagent 2.

Available Applications

Cobas Mira

Cobas Mira-s

Cobas Mira Plus

Hitachi 704

Hitachi 717

Hitachi 737

Hitachi 747

Hitachi 902

Hitachi 904

Hitachi 911

Hitachi 912

Hitachi 917

Hitachi Modular P

Synchron CX 4 / 5 / 7 / 9

Synchron LX20

Unicel DXC 600 / 800